How Do Phytoplankton Adapt to Fluctuating Light? Response of the Photosynthetic Apparatus to Intermittent Exposure to Bright Light

研究表明，在任何给定的条件下， 辐照度在很大程度上取决于时间尺度上的光历史 从几分钟到几小时甚至几天 然而，这些研究都失败了 提供了一个预测性的理解光合作用在变量 光 新技术使我们有可能研究 光合、光保护和光抑制过程 控制光合作用的瞬时速率。 这 技术包括快速重复率（FRR）荧光， 检查光保护、光抑制和光合作用， ~（18）O质谱法测定总光合速率 和氧气在光照下循环。 在实验室中，本研究将校准FRR荧光为基础的 根据测量的总光合速率估计总光合作用 光合作用获得的18 O2交换和量化的作用， 光保护和光抑制在控制 光合作用在野外研究中，原位FRR荧光 测量将用于建立二维（即， 深度和时间）的光化学和非 光化学猝灭系数，计算瞬时 光合作用速率，并检查光保护的作用 和光抑制在控制浮游植物光合作用中的作用 原地。

Studies have demonstrated that photosynthesis rates at any given irradiance depend critically on the light history over time scales of minutes to hours and days. However, these studies have failed to provide a predictive understanding of photosynthesis in variable light. New technology makes it possible to examine the roles of photosynthetic, photoprotective and photoinhibitory processes in controlling the instantaneous rate of photosynthesis. This technology includes fast repetition rate (FRR) fluorescence for examining photoprotection, photoinhibition and photosynthesis, and 18O mass spectrometry for measuring the rate gross photosynthesis and oxygen cycling in the light. In the laboratory, this study will calibrate FRR fluorescence-based estimate of gross photosynthesis against measured rates of gross photosynthesis obtained from 18O2 exchange and quantify the roles of photoprotection and photoinhibition in controlling the rate of photosynthesis. In field research, in situ FRR fluorescence measurements will be used to establish a two-dimensional (i.e. depth and time) description of the photochemical and non- photochemical quenching coefficients, calculate the instantaneous rate of photosynthesis and examine the roles of the photoprotection and photoinhibition in controlling phytoplankton photosynthesis in situ.