Characterization of Eucaryotic Initiation Factor Interactionwith mRNA and mRNA Analogs

9303661 Goss该项目的目标是了解真核蛋白质合成的机制。 特别是一个详细的研究计划的平衡和动力学参数表征的真核起始因子与mRNA和寡核苷酸的相互作用。 具体目标包括：（1）研究起始因子与mRNA上的5 '帽结构的相互作用，（2）研究eIf-4 B与mRNA的相互作用，（3）探索各种起始因子一起和单独与寡核苷酸的相互作用（包括ATP效应），和（4）研究起始因子与寡核苷酸和mRNA相互作用的动力学。 从许多观点来看，蛋白质合成始于起始，这一过程涉及许多蛋白质因子和mRNA的特定部分。 这是一个有争议的研究领域，因为该系统对实验参数和简化的选择（即，使用哪些组件）。 计划中的研究很重要，因为它们将实际量化所涉及的相互作用。 这将最终使比较、理解和整合所有实验室的结果成为可能，前提是实验在开始时就有明确的描述。 ***

9303661 Goss The goal of this project is understanding the mechanism of eucaryotic protein synthesis. In particular a detailed study is planned of equilibrium and kinetic parameters characterizing the interactions of eucaryotic initiation factors with mRNA and oligonucleotides. Specific aims include: (1) study of initiation factor interactions with 5'-cap structures on mRNA, (2) study of the interaction of eIf-4B with mRNA, (3) explore interactions of various initiation factors together and separately with oligonucleotides (including ATP effects), and (4) investigate the kinetics of initiation factor interactions with oligonucleotides and mRNA. %%% Protein synthesis from many points of view begins with initiation, a process involving numerous protein factors and specific portions of mRNA. This is a contentious area of research because the system is so sensitive to experimental parameters and the choice of simplifications (ie. which components to employ). The studies planned are important because they will actually quantitate the interactions involved. This will ultimately make it possible to compare, understand, and integrate results from all labs, providing the experiments are clearly described to begin with. ***