RUI: Immunobiologic Studies of a Marsupial

小行星9305401 本项目的总体目标是启动有袋动物，Monodelphis astritica，灰色，短尾负鼠的分子免疫生物学研究。 已经发现，Monodelphis表现出典型的初级免疫应答，但非典型的次级应答。 其他最近的研究结果表明，典型的真兽目同种型开关从免疫球蛋白M到免疫球蛋白G是缺乏在这个物种。 进一步的研究将比较Monodelphis的免疫反应与小鼠，一个典型的真兽有机体。 与其他实验室合作，将采用分子方法研究主要组织相容性系统和T细胞受体。 这项研究将继续涉及聪明，年轻的本科生。 哺乳动物的免疫系统是人类健康研究的主题，对小鼠和人类的免疫功能了解很多。相比之下，对其他动物自我识别和防御的类似机制知之甚少。 本项目的总体目标是描述有袋动物，Monodelphis arctica，灰色短尾负鼠的免疫反应。 它是美国唯一一种适应实验室的有袋动物。 由于有袋类动物在大约1.3亿年前从真兽目哺乳动物中分离出来，因此对其免疫系统的了解可能有助于了解哺乳动物免疫系统的起源。 将在细胞和生物化学水平进行研究，并将启动分子免疫生物学研究。 在先前的NSF支持下，已经发现Monodelphis表现出典型的初级免疫应答。 进一步的研究将比较Monodelphis的免疫反应与小鼠，一个典型的真兽目哺乳动物。 与其他实验室合作，分子方法将用于鉴定MHC基因，并研究T细胞受体。 这项研究将继续涉及聪明，年轻的本科生。 ***

9305401 Stone The overall objective of this project is to initiate molecular immunobiologic studies of the marsupial, Monodelphis domestica, the gray, short-tailed opossum. It has already been found that Monodelphis exhibits a typical primary immune response but an atypical secondary response. Other recent results suggest that the typical eutherian isotype switch from immunoglobulin M to Immunoglobulin G is lacking in this species. Further studies will compare Monodelphis immune responses with the mouse, a typical eutherian organism. In collaboration with other laboratories, molecular approaches will be used to study the major histocompatibility system and the T cell receptor. This research will continue to involve bright, young undergraduate students. %%% The mammalian immune system is the subject of much research in the context of human health, and much is known about immune function in the mouse and man. In contrast, very little is known about the analogous mechanisms of self recognition and defense in other animals. The overall objective of this project is to characterize the immune response of the marsupial, Monodelphis domestica, the gray, short-tailed opossum. This species is the only laboratory- adapted marsupial in America. Because marsupials separated from eutherian mammals about 130 million years ago, knowledge of their immune system may shed light on the origin of the mammalian immune system. Studies will be carried out at the cellular and biochemical levels and molecular immunobiologic studies will be initiated. With prior NSF support it has been found that Monodelphis exhibits a typical primary immune response. Further studies will compare Monodelphis immune responses with the mouse, a typical eutherian mammal. In collaboration with other laboratories molecular approaches will be used to identify MHC genes, and study the T cell receptor. This research will continue to involve bright, young undergraduate students. ***