Collaborative Research: The Population Genetics of Plant- Fungal Interactions

9220906多年来，杜达什近亲交配抑郁症一直是人们关注的主题。尽管许多研究人员量化了自交产生的后代相对于两个不同个体之间交配产生的后代表现不佳的程度，但很少有实证研究关注自然异交率不同的群体在近亲繁殖衰退方面的差异。这项研究的主要目的是确定近亲繁殖抑制在加利福尼亚州北部两个密切相关的本土物种中表达的潜在遗传基础，这两个物种是自花授粉的微花水母和依赖昆虫(从而异交)的水母。目标有三个方面。首先，根据自交和异交物种的两个群体的雄性和雌性功能来量化近交衰退的程度。近亲交配对女性生殖功能的影响是有充分证据的，但对男性功能的影响，如花粉产生，在很大程度上是未知的。其次，将通过两个温室育种实验来量化近交衰退的遗传基础。第三，近亲繁殖抑制的家系间差异将作为观察到的花的变化的函数在异交滴虫中被量化。这两个物种的比较将研究交配系统和所观察到的近亲繁殖衰退的遗传基础之间的关系，从而提供关于自然植物种群交配系统变化的遗传学驱动的基线信息。对这两个相关物种的研究结果将为保护作物基因库多样性和稀有物种遗传多样性提供见解。

9220906 Dudash Inbreeding depression has been the subject of considerable attention for over 100 years. Although many researchers have quantified how progeny generated by self-matings perform poorly relative to progeny generated from matings between two different individuals, few empirical studies have focused on the differences in inbreeding depression in populations that differ in their natural outcrossing rates. The main goal of the proposed research is to determine the underlying genetic basis of the expression of inbreeding depression in two closely related native species from northern California, the self-pollinating Mimulas micranthus and the insect dependent (thus outcrossing) M. guttatus. The objectives are three-fold. First, to quantify the degree of inbreeding depression in terms of both male and female function in two populations of both the selfing and outcrossing species. The effects of inbreeding on female reproductive function are well documented, but he effects on male function, such as pollen production are largely unknown. Second, the genetic basis of inbreeding depression will be quantified by two greenhouse breeding experiments. Third, among-family level variation in inbreeding depression will be quantified in the outcrossing M. guttatus as a function of the observed floral variation. This two-species comparison will examine the relationship between the mating system and the genetic basis of the observed inbreeding depression, thus providing baseline information on the genetics driving changes in mating systems in natural plant populations. The results generated from the research on these two related species will provide insights into preserving both crop gene pool diversity and rare species genetic diversity.***