RPG: Molecular Mechanisms for the Bone Response to Mechanical Loading in an in vivo Model: Preliminary Studies

9306921 Bloomfield这项针对女性的研究计划资助支持研究胰岛素样生长因子II （IGF-II）在介导骨对机械应变变化的反应中的潜在作用。先前的研究人员已经开发了有价值的动物模型，以量化和详细描述骨对骨所经历的负荷增加以及各种固定治疗的负荷减少的骨反应的性质。然而，对于在这些情况下通常观察到的骨体积增加或减少的细胞或分子机制知之甚少。具体来说，骨细胞如何感知张力，然后将机械信号转导到生化因子，然后启动骨形成活动的增加（在负荷增加的情况下）或抑制形成以有利于吸收（在负荷减少的情况下）？本提案的规划活动将提供对这条研究路线至关重要的两种主要工具的培训：骨组织形态学和分子生物学技术，用于从骨组织中分离和量化IGFs的mRNA。＊＊＊

9306921 Bloomfield This research planning grant for women supports studies exploring the potential role of insulin-like growth factor II (IGF-II) in mediating the response of bone to changes in mechanical strain. Previous investigators have developed valuable animal models to quantify and describe in detail the nature of the bony response to increases in loading experienced by bone, as well as to the decreased loading of various immobilization treatments. Little is known, however, about cellular or molecular mechanisms for the subsequent gain or loss in bone volume routinely observed with these conditions. Specifically, how do bone cells perceive strain and then transduce that mechanical signal to a biochemical factor which then initiates increases in bone formation activity (in the case of increased loading) or suppresses formation in favor of resorption (in the case of decreased loading)? The planning activities of this proposal will provide training in two primary tools essential to this line of research: bone histomorphometry and molecular biology techniques to isolate and quantify mRNA for IGFs from bone tissue. ***