The Screening of Mutagenized abi3 Seeds of Arabidopsis thaliana for New Giberellin Sensitivity Mutants, and their Characterization

9307067海绵这项拟议的研究涉及一种新的诱变拟南芥种子筛选程序，以选择在合成或响应植物激素的Bibberellin(GA)组的能力上不同的植物身高突变体。由于通常需要GAs来对抗脱落酸(ABA)诱导的拟南芥种子休眠，所以最初的诱变将使用AB13座位上纯合子隐性的种子进行。AB13等位基因导致对ABA不敏感，从而防止种子休眠。预计扰乱生物合成或对GA反应的突变，通常会阻止种子萌发，不会损害AB13种子的萌发。因此，这种筛选方法应该允许分离新的GA突变体，特别是新的GA响应突变体。在该奖项的12个月期间，建议与M.埃斯特尔博士一起开始对这些突变体的基因分析。生化分析也将启动。将采用气相色谱-质谱仪相结合的方法对新突变株中的GAS进行鉴定和鉴定。新的GA不敏感突变体的特征将有助于鉴定参与这些事件的基因。在这些突变体中，GA信号的接收或该信号向生长反应的转导受到损害。

9307067 Sponsel The proposed research involves a new screening procedure for mutagenised Arabidopsis thaliana seeds to select plant stature mutants which differ in their ability synthesize or respond to the bibberellin (GA) group of plant hormones. Since GAs are normally required to counteract abscisic acid (ABA)-induced seed dormancy in Arabidopsis the original mutagenesis will be conducted using seeds which are homozygous recessive at the AB13 locus. The ab13 allele causes insensitivity to ABA and hence prevents seed dormancy. It is expected that mutations which perturb the biosynthesis or response to GA, and which would normally prevent seed germination, will not impair germination in ab13 seeds. Therefore this screening protocol should allow the isolation of new GA mutants, especially new GA-response mutants. During the twelve months of this award it is proposed to begin the genetic analysis of these mutants in association with Dr. M. Estelle. The biochemical analysis will also be initiated. It will involved the identification and qualification of GAs in the new mutants using combined gas chromatography-mass spectrometry. The characterization of new GA-insensitive mutants in which the reception of the GA signal, or the tranduction of that signal into a growth response is impaired will assist in the identification of genes which are involved in these events.