Regulation and Function of a Maize Ribosome-Inactivating Protein (RIP)

9307979波士顿这是一项关于玉米核糖体失活蛋白的研究。RIPs通过去除rRNA中的腺嘌呤A4324来催化灭活核糖体。这就导致了对翻译的抑制。玉米PRI是已知的唯一以非活性前体形式积累的单链RIP基因产物。具体目标是确定ProRIP加工和RIP活性所需的蛋白质结构域，并将核酸结合从催化结构域中分离出来。核糖体失活蛋白(RIPs)存在于玉米、小麦和大麦等多种植物的种子、根、叶或汁液中，具有极强的细胞毒性，可起到防御和储存食物的作用。了解这些蛋白质的激活和生理作用对于开发包括杀虫剂和杀菌剂在内的选择性细胞杀伤剂非常重要。***

9307979 Boston This is a study of the maize ribosome inactivating protein (RIP). RIPs catalytically inactivate ribosomes by removing adenine A4324 in rRNA. This leads to inhibition of translation. Maize PRI is the only known single chain RIP gene product that accumulates as an inactive precursor. The specific objectives are to determine the protein domains required for proRIP processing and RIP activity, and to separate the nucleic acid binding from the catalytic domain. %%% Ribosome inactivating proteins (RIPs), which occur in the seed, root, leaf, or sap of a wide variety of plants including maize, wheat, and barley are extremely cytotoxic, and may serve defensive as well as food storage roles. Understanding activation and the physiological role of these proteins are important for development of selective cell-killing agents, including insecticides and fungicides. ***