Application of Adhesion-Deficient VOC-Degrading Bacteria in the Bioremediation of Contaminated Aquifers

WPCM 2 B P Z Courier 10cpi #| x =V x 6 x @ 8；X @佳能LBP-8III（附加）calb8ad。PRS x @ 0jzx @ #| x 2 zb % x F '佳能LBP-8III（附加）calb8ad。PRS x @ 0jzx @信使10cpi信使脚注16cpi。？ x x x, x 6 x @ 8；X @ + h h h, s, h 6 X @；@ t" t} r} r 6e t} r 6h t} r。原位生物修复要求有能力的降解细菌能够穿透土壤或含水层固体到达污染物。这种方法的主要障碍之一是细菌通常会牢固地附着在固体表面，堵塞多孔材料而不是穿过它们。我们的第一阶段研究证明了分离几种不同细菌菌株的非粘附变体的可行性，这些菌株能够降解地下水和含水层中常见的一组氯化有机化学品污染物。在第一阶段开发的菌株比其粘附的亲本菌株至少有一个数量级进入含水层固体的潜力。通过使用三氯乙烯作为模型氯化有机，我们也证明了这些非粘性变体的降解能力没有改变。第二阶段的研究将通过建立一些操作参数来实现该技术的商业化，这些操作参数用于这些非粘性降解菌株的原位使用。除了建立粘附缺陷表型的稳定性外，我们还将测量这些菌株在不同类型含水层材料中的渗透和降解效率。将建立一个中试规模的模型含水层，并用于演示基于使用这些高度专业化的微生物的原位生物修复系统。地下水污染在世界范围内是一个日益严重的问题，发展控制它的方法是极其重要的。解决这个问题的一种方法是继续抽水并在表面进行净化。由于这是非常昂贵的，人们正在寻求新的方法，通过在井中或在含水层中使用微生物来破坏污染水的化学物质。这些细胞可以消耗污染的化学物质并净化水。然而，在这个过程中，降解污染化合物的细胞会附着在土壤颗粒上，随着它们代谢污染物，它们会生长，最终堵塞水井。为了避免这个问题，我们开发了一些污染物降解、不粘附或不粘附的细菌菌株。在目前的研究项目中，我们将确定这些非黏附细胞在地下水净化中是否同样有效，但不会成长为屏障并堵塞水流入井中。*** 0 * 0 * 0 *

WPCM 2 B P Z Courier 10cpi #| x =V x 6 X @ 8 ; X @ Canon LBP-8III (Additional) CALB8IAD.PRS x @ 0J zX @ #| x 2 Z B % X F ` Canon LBP-8III (Additional) CALB8IAD.PRS x @ 0J zX @Courier 10cpi Courier Footnote 16cpi . ? x x x , x 6 X @ 8 ; X @ + H H H , S , H 6 X @ ; @ t" t } r } r 6e t t } r 6h t } r . s u t a s } r' I ut r PQV 6e L 2 K 9310770 DeFlaun In situ bioremediation requires that competent degradative bacteria be able to penetrate soil or aquifer solids to reach the contamination. One of the major obstacles to this approach is that bacteria often adhere strongly to solid surfaces and plug porous materials rather than pass through them. Our Phase I research demonstrated the feasibility of isolating non adhesive variants of several different bacterial strains that are capable of degrading a suite of chlorinated organic chemicals commonly found as contaminants in groundwater and aquifers. The strains that were developed in Phase I have the potential to travel at least an order of magnitude further into aquifer solids than their adhesive parental strains. By using trichloroethylene as a model chlorinated organic, we also demonstrated that the degradative competence of these non adhesive variants had not been altered. Phase II research will bring this technology to commercialization by establishing a number of operating parameters for the use of these non adhesive degradative strains in situ . In addition to establishing the stability of the adhesion deficient phenot ype, we will measure the penetration and degradative efficiency of these strains in different types of aquifer material. A pilot scale model aquifer will be constructed and used to demonstrate an in situ bioremediation system based on the use of these highly specialized microorganisms. %%% Ground water pollution is a growing problem worldwide and the development of methods to control it are extremely important. One method to confront this problem is to continue to pump water and purify it on the surface. Since this is very expensive, new methods are being sought to destroy water contaminating chemicals by using microorganisms in the well, or in the aquifer, itself. These cells can consume contaminating chemicals and purify the water. However, during the process, the cells that degrade the polluting compounds stick to soil particles, and, as they metabolize pollutants, they grow and eventually plug the well. In an attempt to circumvent this problem, we developed certain strains of pollutant degrading, non sticking, or non adhering, bacteria. In the present research program, we will determine if these non adhering cells work equally well in ground water purification, but without growing into a barrier and plugging the flow of water into the well. *** 0*0*0*