Development of the Dynamic Clamp

此SGER提案旨在完成“动态钳”的开发，这是一个用于神经元和神经元电路在线电生理分析的软件工具。目前的微电极技术可以记录神经元内在活动和注入电流对膜电位变化的响应，也可以记录电压箝位下的局部膜电流。动态钳为神经元和网络响应的记录和实验操作提供了重大进展，使其能够在实验过程中实时模拟神经元的诱发和电压依赖性电导变化。这使得模拟两个神经元之间的电或化学突触连接，模拟神经调节剂物质的作用，以及模拟电压依赖的膜电流的作用成为可能。电流或突触可以在神经元中增加、减少或改变，这些操作的效果可以直接评估。这种支持将使pi能够完成软件的开发和测试，包括“用户友好”界面。P.I.已经通过最新一期《神经生理学杂志》（Journal of Neurophysiology）的封面图片和一份关于其使用情况的报告，提醒了公众动态钳的存在。P.I.还建立了软件的分发和技术支持机制

This SGER proposal is to complete development of the "Dynamic Clamp" which is a software tool for on-line electrophysiological analysis of neurons and neuronal circuits. Present microelectrode techniques make it possible to record membrane potential changes in response to intrinsic neuronal activity and to injected current, and to record local membrane currents under voltage clamp. The dynamic clamp provides a significant advance for recording and experimental manipulation of neuronal and network responses by making it possible to simulate evoked and voltage-dependent conductance changes in a neuron in real time during an experiment. This makes it possible to simulate an electrical or chemical synaptic connection between two neurons, to simulate the effect of a neuromodulator substance, and to simulate the effect of voltage- dependent membrane currents. Currents or synapses can be added or subtracted or changed in a neuron, and the effect of these manipulations can be directly assessed. This support will enable the P.I. to complete the development and testing of the software, including a "user-friendly" interface. The P.I. has already alerted the community to the existence of the Dynamic Clamp through a cover picture on the current issue of the Journal of Neurophysiology, and a report on its use. The P.I. has also established mechanisms for distribution and technical support of the software.***//