Acquisition of a Kodak Ektapro EM 1012 Motion Analyzer System

9318129 Biewener动物运动的准确成像是研究动物运动的神经肌肉控制以及动物功能的生物力学和流体动力学的基础。动物节段或动物本身运动的定量运动学分析可以与同时记录神经放电、肌肉激活、力的产生和缩短、骨骼应变或通过动物或动物周围的流动模式的可视化有关。通常，尤其是对小动物来说，这些运动以非常快的速度和很短的时间间隔进行。要以合理的时间分辨率记录这些快速运动，可能需要大于1000 Hz的成像速率。高速胶片分析是P.I.的核心组成部分和三个合作伙伴。我的研究。多年来，我们一直依靠16毫米胶片的记录和分析来进行这样的研究，以获得高达500帧每秒的成像速率。高速摄像技术的最新发展使得使用基于视频的记录和分析系统获得更高的成像速率成为可能。因此，本提案要求拨款购买一套高速录像系统，作为芝加哥大学生物与解剖学系的多用户设备。我们寻求购买的设备是柯达Ektapro EM 1012运动分析系统，配备了强化成像相机和分析工作站。该系统能够在弱光条件下获得1000全帧每秒，产生出色的景深（对动物运动模式的可靠监测至关重要）。通过划分每个帧映射到的存储区域，可以获得高达6000张图像秒-1的实际成像速率。这个系统是最先进的，很可能会保持很多年。最近，从事这些生物学领域工作的其他实验室也收购了柯达Ektapro及其竞争系统。为了保持与这些团体的竞争力，并提供资源，以吸引希望攻读生物力学和神经肌肉生物学研究生培训的学生，我们认为获得这样一个系统是至关重要的。虽然该系统的成本太高，不足以证明单个研究者的建议是合理的，但它适合作为共享设施使用。该系统非常适合研究人员/实验室小组（1至2周）的短期密集使用，在此期间，可以记录大量的运动学数据，并在研究人员自己的实验室将数字化图像文件下载到可移动高密度磁盘媒体上后进行分析。我们的目标是不仅在我们的每个实验室之间共享这个高速视频系统，而且将其提供给大学其他单位的学生和研究人员。我们也设想限制大学以外的人进入。私家侦探将监督设备的调度、使用和维护。

9318129 Biewener Accurate imaging of animal movements underlies studies of the neuromuscular control of animal movement and the biomechanics and hydrodynamics of animal function. Quantitative kinematic analyses of the movements of animal segments or of the animal itself can be related to simultaneous recordings of nerve firing, muscle activation, force generation and shortening, skeletal strain, or to visualization of flow patterns through or around the animal. Often, and especially for small animals, these movements occur at very rapid rates and over short time intervals. To record these rapid movements with reasonable time resolution can require imaging rates of greater than 1000 Hz. High-speed film analysis represents a central component of the P.I.'s and the three Co-P.I's research. For many years, we have carried out such studies by relying on 16 mm film recording and analysis to obtain imaging rates as high as 500 frames sec-1. Recent developments in high speed videography now make it possible to obtain even higher imaging rates using a video based recording and analysis system. This proposal therefore requests funds to purchase a high-speed video system to be installed as a multi-user facility within the Department of Organismal Biology & Anatomy at the University of Chicago. The equipment that we seek to purchase is a Kodak Ektapro EM 1012 Motion Analyzer System, equipped with an intensified imaging camera and analysis workstation. This system is capable of obtaining 1000 full frames sec-l under low light conditions, yielding excellent depth of field (essential to reliable monitoring of animal movement patterns). By partitioning the memory area onto which each frame is mapped, practical imaging rates of up to 6000 images sec-1 can be obtained. This system is state-of-the-art and is likely to remain so for many years. The Kodak Ektapro and competitive systems have been recently acquired by other labs working in these areas of biology. To remain competit ive with these groups and to provide a resource that will attract students who wish to pursue graduate training in biomechanics and neuromuscular biology, we believe that it is essential that we obtain such a system. While the cost of the system is too high to justify on a single investigator proposal, it is appropriate for use as a shared facility. This system is ideal for short-term, intensive use by an investigator/lab group (1 to 2 weeks), during which time considerable kinematic data can be recorded and later analyzed in the investigator's own lab after downloading the digitized image files onto removable high-density disk media. Our goal is to not only share this high-speed video system among each of our labs, but to make it available to students and research faculty in other units within the University. We also envision limited access by persons outside the University. The P.I. will oversee scheduling, use and maintenance of the equipment.