Phospholipid Acylation in Cotyledons of Cotton Seedlings

查普曼9320047磷脂是所有生物膜不可或缺的组成部分。细胞内膜的磷脂组成变化很大。磷脂组成的不同决定了膜的特性、物理特性和功能。磷脂的零售，如磷脂链的脱酰化或酰化，可能是细胞快速调整膜功能以响应环境条件变化的重要机制，如温度、盐浓度、水分活度等。尽管人们对膜脂代谢作为保护环境胁迫的一种方式的作用越来越感兴趣，但现有膜磷脂的零售机制受到的关注较少。这项研究的目的是研究生化途径(S)的作用，它支持将酰基部分结合到现有的植物膜磷脂中。这是通过一种不同于普通植物从新合成磷脂的生物合成途径来完成的。这项研究计划的具体目的是描绘导致这种不寻常的酰基掺入预制棉籽膜磷脂的生化途径(S)，并确定参与这一过程的酶(S)。这是一个基础研究项目，目的是确定植物细胞如何有效地调节其膜的化学成分，以抵御环境中的破坏性变化，如温度、盐浓度、水分活度等。尽管环境胁迫对膜形成中脂质生物合成的影响已经成为一段时间以来解释植物抗性发展的研究主题，但现有膜中磷脂化学的变化还没有。将进行实验，以确定植物如何改变膜磷脂的结构，并确定涉及的酶。这项研究将大大增加我们对植物对环境胁迫的抗性的理解和能力。***

Chapman 9320047 Phospholipids are an integral component of all biological membranes. The phospholipid composition of intracellular membranes is highly variable. Differences in phospholipid composition determine the specific properties of the membrane; its physical properties and its function. Retailoring of phospholipids, such as deacylation or acylation of phospholipid chains in existing membranes, may be an important mechanism that the cell has for rapidly adjusting membrane function in response to changing environmental conditions, such as temperature, salt concentration, water activity, etc. Although there has been growing interest in the role of membrane lipid metabolism as a way to protect against environmental stress, retailoring mechanisms of existing membrane phospholipids has received less attention. The aim of this research program is to examine the role of biochemical pathway(s) that support the incorporation of acyl moieties into existing plant membrane phospholipids. This is accomplished by a pathway different from the ordinary plant biosynthetic pathway for de novo phospholipid synthesis. The specific aim of this research program is to delineate the biochemical pathway(s) leading to this unusual acyl-group incorporation into preformed cottonseed membrane phospholipids and to identify the enzyme(s) involved in the process. %%% This is a basic research project to determine how plant cells can efficiently regulate the chemical composition of their membranes to resist destructive changes in their environment, such as temperature, salt concentrations, water activity, etc. Although the effects of environmental stress on lipid biosynthesis in membrane formation have been a subject of research for some time to explain the development of plant resistance, changes in phospholipid chemistry in existing membranes has not. Experiments will be conducted to determine how plants can change the structure of membrane phospholipids and identify the enzymes involved. This research should significantly increase our understanding and ability to control resistance to environmental stress in plants. ***