Nitrification and Denitrification: Transformation Rates and Molecular Probes for Bacteria in the Nitrogen Cycle

9402538 Ward本课题将结合传统的15N示踪方法和免疫探针来研究海洋最小氧区与反硝化和硝化之间的关系，以表征参与转化的细菌组合的分布。研究地点位于东热带北太平洋（ETNP），将呈现一系列条件，包括含氧良好的地表水，不同强度的氧极小值和海洋深水。由于改进的同位素探针可以进行小规模采样和更小体积的孵育，研究人员将在接近化学变量可能的规模上研究细菌学特性。这将允许确定在ETNP的氧条件光谱中硝化和反硝化重叠和相互作用的程度。

9402538 Ward This proposal will investigate the relationship between the oceanic oxygen minimum zone and denitrification and nitrification using a combination of traditional 15N tracer methods and immunological probes t characterize the distribution of the bacterial assemblages involved in the transformations. The study site in the Eastern Tropical North Pacific (ETNP) will present a range of conditions including well oxygenated surface waters, oxygen minima of varying intensities, and oceanic deep water. Due to the small scale sampling and smaller volume incubations made possible by improved isotope probes, the investigators will study the bacteriological properties on a scale approaching that possible for chemical variables. This will allow a determination of the extent to which nitrification and denitrification overlap and interact in the spectrum of oxygen conditions of the ETNP.