Oak Tannins, Gypsy Moths, and LdNPV: Anti-Viral Activity Depends on Oxidation and Covalent Binding of Gallotannins to Viral Proteins

小阿佩尔9407308 植物化学单宁在生态相互作用中的高度可变的活性导致了对其生态意义的相当大的争议。看来，这种变化和争议是由于未能认识到氧化在活化这些分子中的重要性。也就是说，植物中的化学物质接受来自单宁的电子，这反过来又促进了单宁在昆虫肠道中作为抗病毒剂的作用。 在这项研究中，电子捐赠和化学键合的重要性将通过证明氧化单宁行动的重要性，通过测定单宁的抗病毒活性在存在和不存在的植物氧化剂，并证明这些氧化单宁化学结合到病毒蛋白质的模型肠道系统的吉普赛蛾。 ***

9407308 Appel The highly variable activity of plant chemical tannins in ecological interactions has led to considerable controversy about their ecological significance. It appears that this variation and controversy arise from a failure to realize the importance of oxidation in activating these molecules. That is, chemicals in the plant accept electrons from tannins and this in turn facilitates the action of tannins as anti-viral agents in the insect gut. In this study the importance of electron donation and chemical bonding will be investigated by demonstrating the importance of oxidation to tannin action by assaying the antiviral activity of tannins in the presence and absence of plant oxidants and by demonstrating that these oxidized tannins bind chemically to viral proteins in the model gut system of the Gypsy Moth. ***