New Strategies for the Production of Efficient Catalytic Antibodies

9412502 Georgiou该项目是关于研究开发催化抗体生产的新策略。 这些策略是基于大量不同抗体在E. coli细胞表面，然后进行筛选程序以从群体中选择所需的分子。 选择过程将利用过渡态类似物的荧光标记缀合物与细胞表面上的抗体之间的可能结合。 然后可以通过荧光激活细胞分选（FACS）选择与过渡态类似物结合的细胞。 在一项平行研究中，本研究将试图证明直接基于产物形成速率而不是结合过渡态类似物的催化抗体的分离。 催化性抗体具有高度的选择性和活性，但由于分离和生产的高成本，目前对于商业目的来说过于昂贵。 该项目的一部分是关于降低这一成本的新方法。 ***

9412502 Georgiou This project is on research to develop new strategies for the production of catalytic antibodies. The strategies are based on the expression of a vast number of different antibodies on the E. coli cell surface followed by screening procedures for selecting the desired molecules from the population. The selection process will make use of the possible binding between the fluorescently labeled conjugate of the transition state analog and the antibody on the cell surface. Cells bound to the transition state analog can then be selected by fluorescent activated cell sorting (FACS). In a parallel study, this research will attempt to demonstrate the isolation of catalytic antibodies directly based on the rate of product formation rather than binding to transition state analogs. Catalytic antibodies have a high degree of selectivity and activity, but are currently prohibitively expensive for commercial purposes because of the high cost of isolation and production. Part of this project is on new approaches to lower this cost. ***