U.S.-Poland Cooperative Science: Design and Chemistry of Modified Nucleosides for Nucleic Acid Synthesis

9412482 AGRIS这个由北卡罗来纳州立大学的保罗·F·阿格里斯博士和罗兹工业大学的安德烈·马尔凯维奇博士共同开展的美国-波兰研究项目的题目是“用于核酸合成的修饰核苷的设计和化学”。修饰的核苷的化学、结构和动力学如何与RNA的功能有关，也与DNA有关，目前还不清楚。一旦了解，即使对于少量碱基修饰的核苷，这种不同的化学和结构集合也可以应用于对生物和医学重要的分子的设计和工程。RNA和DNA中80多个修饰核苷的进化与它们的位点选择定位和核酸化学和结构的改变有关；实现这一目标的可能性与修饰的化学和结构一样多种多样。合作的研究人员将开发新的技术和方法，研究在RNA和DNA中选择性地结合天然和非天然修饰和稳定同位素标记的核苷，最初适用于基础核酸生物化学(分子进化、核酸结构)。合作项目的互惠互利源于两位调查人员的完美互补经验。Malkiewicz博士在修饰核苷和含有修饰核苷的小低聚物的有机化学合成、化学和分析方面具有丰富的经验；而AGRIS博士在RNA中修饰的核苷的结构和功能的生物合成和分析方面具有丰富的经验。这个生物化学项目通过使波兰和美国的顶尖专家能够将互补的人才结合在一起，并在相互感兴趣和能力很强的领域汇集资源，从而实现了促进科学知识的计划目标。***

9412482 Agris This US-Poland Research project between Dr. Paul F. Agris of North Carolina State University, and Dr. Andrzej Malkiewicz of the Technical University of Lodz, is on "Design and Chemistry of Modified Nucleosides for Nucleic Acid Synthesis." How modified nucleoside chemistry, structure and dynamics relate to function of RNAs, but also DNA, is not understood. Once understood, even for a small number of base modified nucleosides, this varied collection of chemistries and structures can be applied to the design and engineering of molecules important to biology and medicine. The evolution of the more than 80 modified nucleosides in RNAs and DNA is connected with their site-selected positioning and altering of nucleic acid chemistry and structure; the potential to accomplish this is as varied as are the chemistries and structures of the modifications. The cooperating investigators will develop new technologies and methods for studying the site-selected incorporation of both native and non- native modified and stable-isotope labeled nucleosides in RNA and DNA, applicable, initially, to fundamental nucleic acid biochemistry (molecular evolution, nucleic acid structure). The mutual benefit from the cooperative project derives from the perfect complementary experiences of the two investigators. Dr. Malkiewicz is broadly experienced in the organic chemical syntheses, chemistry and analyses of modified nucleosides and small oligomers containing modified nucleosides; whereas Dr. Agris is broadly experienced in the biosynthesis and analyses of both the structures and functions of modified nucleosides in RNA. This project in biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in Poland and the United States to combine complementary talents and pool resources in areas of strong mutual interest and competence. ***