Legume Cell Cycle Genes to Monitor Stress (Salinity and HighTemperature) Response During Early Nodulation, U.S.-Pakistan Cooperative Research

项目描述：本项目支持美国田纳西大学诺克斯维尔分校的Peter Gresshoff博士和巴基斯坦费萨拉巴德国家生物技术与基因工程研究所（NIBGE）所长Kauser Malik博士的联合项目。本研究旨在了解热、盐胁迫条件下豆科植物结瘤的影响机制。这项工作是寻找更多关于这些压力的细胞目标的信息。由于结瘤是共生固氮的先决条件，因此工作将集中在这一初始步骤上。结瘤可能是在G2阻滞的皮质细胞中通过细胞分裂开始的。计划要求使用分子探针和PCR引物来检测最近在Gresshoff实验室从大豆中分离出的两个细胞周期基因（即cyclin-B和cdc2）。重点将放在非放射性PCR技术上，以确定胁迫如何调节这些关键基因的表达。该合作项目的目标是：将技术从大豆转移到绿豆系统；培训费萨拉巴德研究组使用分子探针和引物评估热盐胁迫对绿豆早期结瘤的影响；确定大豆和绿豆基因排列的进化差异程度；进一步发展细胞周期参与分生组织起始和维持的知识基础。范围：该项目符合国际项目司的一些重要目标：它允许两位具有互补能力的受人尊敬的科学家合作进行重要的科学工作；它允许发展中国家的外国科学家积累必要的经验，以便在这个项目之后继续进行这项有用的研究；它还有助于了解影响世界各地边缘土地生产力的问题的原因。这位巴基斯坦科学家是该国该领域最杰出的研究人员之一，拥有令人印象深刻的科学出版物记录。由于他的工作，NIBGE最近成立了，并由他担任首任主任。＊＊＊

9413248 Gresshoff Description: This project supports a joint project by Dr. Peter Gresshoff, of the University of Tennessee, Knoxville, and Dr. Kauser Malik, Director of the National Institute of Biotechnology and Genetic Engineering (NIBGE) in Faisalabad, Pakistan. The research aims at understanding the mechanisms affecting the nodulation in legumes planted under stress conditions such as heat and salinity. The work is to seek more information concerning the cellular targets of these stresses. As nodulation is a prerequisite of symbiotic nitrogen fixation, the work will focus on this initial step. Nodulation starts through cell division possibly induced in G2 arrested cortical cells. Plans call for using molecular probes and PCR primers for two cell-cycle genes (namely cyclin-B and cdc2) which were recently isolated from soybean in Gresshoff's laboratory. Focus will be on non-radioactive PCR techniques to define how stresses modulate the expression of these critical genes. The objectives of this cooperative project are: to transfer technology from soybean to the mungbean system; train the research team in Faisalabad to use molecular probes and primers to evaluate the impact of heat and salinity stress on early nodulation of mungbean; determine the degree of evolutionary divergence between the soybean and mungbean gene arrangements; and further develop the knowledge base of cell cycle involvement in meristem initiation and maintenance. Scope: This project meets a number of important objectives of the Division of International Programs: it allows cooperation of two respected scientists, with complementary capabilities, to do important scientific work; it allows a foreign scientist in a developing country to build the experience necessary to continue this useful research beyond this project; and it helps understand the causes of a problem that affects productivity in marginal lands around the world. The Pakistani scientist is one of that c ountry's most distinguished researchers in the field, with an impressive record of scientific publications. As a result of his work, the NIBGE was recently established, with him as its first director. ***