Manipulating Intracellular Electron Carriers to Accelerate Microbial Detoxification Requiring Monooxygenation Reactions

9413824 Rittmann这是一笔用于支持研究的拨款，其目的是确定在稳定的悬浮生长生物反应器中加速二次底物单氧反应的条件，同时实现其他过程目标，这些生物反应器用于处理受污染的废水、垃圾填埋场的渗滤液和受氯化脂肪族和芳香族有机物质污染的地下水。本课题拟研究的具体底物为4-氯苯酚和2,4二硝基苯酚。将采用两个阶段的过程，其中第一个反应器将用于用减少的电子载体丰富细胞，第二阶段用于确保去除能够在生化上对氧气产生需求的物质，以控制生物量的生长并为生物活性固体提供足够的保留时间。石油源芳烃、氯化脂肪族溶剂和硝基芳烃是美国最常检测到的水中有机污染物。这些化合物的生物降解是一种特别有前途的方法，因为如果成功，降解是完全的，并且在发生降解的物理条件不极端的条件下可能具有经济可行性。该项目的研究结果有望在环境工程实践中对生物反应器的设计进行改进。＊＊＊

9413824 Rittmann This is a grant to support research the objective of which is to determine conditions under which monooxygenation reactions of secondary substrates can be accelerated while achieving other process goals in stable, suspended growth biological reactors that are used to treat contaminated wastewaters, leachates from landfills and groundwaters contaminated by chlorinated aliphatic and aromatic organic substances. The specific substrates the investigator plans on studying in this project are 4-chlorophenol and 2,4 dinitrophenol. A two stage process will be used in which the first reactor will be used to enrich the cells with the reduced electron-carrier and the second stage used to ensure removal of substances capable of biochemically exerting a demand for oxygen, to control growth of biomass and to provide adequate retention time for the biologically active solids. Aromatic hydrocarbons of petroleum origin, chlorinated aliphatic solvents and the nitroaromatics are the most commonly detected organic contaminants of water in the United States. Biological degradation of these compounds is an especially promising approach because, if successful, degradation is complete and likely to be economically viable under conditions that are not extreme regarding the physical conditions under which degradation takes place. Results of this project are expected to improve the design of biological reactors as they are used in environmental engineering practice. ***