Acquisition of HPLC System

这是关于购置高效液相色谱系统的共用设备提案。HPLC通过增加分辨率、提高产率和减少洗脱时间和体积提供了对常规色谱的重大改进。由于可用色谱柱的多样性，HPLC分离几乎适用于任何类别的化合物，包括小有机分子、脂质、核酸或大分子如蛋白质。 所要求的HPLC由提供全功能和高度通用的半制备系统所需的基本组件组成。该装置包括一台IBM 486计算机，该计算机具有用于控制各个HPLC组分和数据分析的适当软件;一个能够形成梯度的单个双溶剂泵系统;一个手动进样器;二极管阵列检测器用于监测各种分子的洗脱二极管阵列允许用户在实验期间扫描多个波长，这将允许研究人员确定洗脱部分是否纯; Quick Res软件用于分析共洗脱化合物;以及HPLC柱的初始分类，以允许基于不同物理性质的分离。 所要求的仪器将增强和现代化的设施，可用于生物化学，微生物学和分子生物学系的研究和教学，除了提供给其他教师和学生以外的部门。HPLC系统将直接受益于几个教师的研究项目，如：1）四环素和烃类的代谢和降解的细菌; 2）调节哺乳动物蛋白水解酶的机制，在体内和体外; 4）翻译后修饰作为一种调节机制，在信号转导过程中的网柄藻; 5）酚类和激素的纯化; 6）研究针对多精受精和海藻胚胎胁迫耐受性的中间和缓慢阻断; 7）在多种真细菌、真核生物和植物中调节膜脂质和脂肪酸组成以及这些分子的代谢。缅因州大学生物化学、微生物学和分子生物学的研究生和本科生课程包括几门实验室课程，这些课程也将通过HPLC系统的可用性得到增强。拟定用途包括纯化免疫学用单克隆抗体;分离和离析分子生物学和细菌遗传学用DNA和聚合酶链反应产物;纯化生物化学中的蛋白质和肽图谱;以及表征细菌生理学用几种微生物的膜脂。在这些课程中加入HPLC将为我们的学生提供使用当前方法的重要经验。这些技能是普遍需要的，对于确保他们在研究生学习，研究工作或职业生涯中的竞争力非常有益。

This is a shared equipment proposal for the acquisition of a high performance liquid chromatography system (HPLC). HPLC provides a major improvement over conventional chromatography by increasing resolution, improving yields, and decreasing elution times and volumes. HPLC separations are applicable to virtually any class of compound, including small organic molecules, lipids, nucleic acids or macromolecules such as proteins, due to the diversity of available columns. The requested HPLC consists of the basic components required to provide a fully functional and highly versatile semi-preparative system. The unit includes an IBM 486 computer with the appropriate software for control of the individual HPLC components and data analysis; a single two solvent pump system capable of gradient formation; a manual sample injector; a Diode array detector for monitoring elution of a wide variety of molecules the Diode array permits the user to scan multiple wavelengths during an experiment, which will allow the researcher to determine if the eluted fraction is pure or not ; the Quick Res software for analysis of co-eluting compounds; and an initial assortment of HPLC columns to allow separations based on different physical properties. The requested instrument will enhance and modernize facilities available for research and teaching in the department of Biochemistry, Microbiology and Molecular Biology in addition to being available to other faculty and students outside of the department. The HPLC system will directly benefit several faculty research projects such as: 1) the metabolism and degradation of tetracycline and hydrocarbons by bacteria; 2) mechanisms regulating mammalian proteolytic enzymes, in vivo and in vitro; 4) post-translational modification as a regulatory mechanism in signal transduction processes in Dictyostelium; 5) purification of phenolics and hormones; 6) studies of the intermediate and slow blocks against polyspermy and embryonic stress tolerance in f ucoid algae and 7) the regulation of membrane lipid and fatty acid composition and the metabolism of these molecules in a variety of eubacteria, eukaryotes and plants. The graduate and undergraduate curricula in Biochemistry, Microbiology and Molecular Biology at the University of Maine include several laboratory courses that will also be enhanced by the availability of a HPLC system. Proposed uses include purification of monoclonal antibodies for Immunology; separation and isolation of DNA and polymerase chain reaction products for Molecular Biology and Bacterial Genetics; purification of proteins and peptide mapping in Biochemistry; and characterization of membrane lipids from several microorganisms for Bacterial Physiology. The inclusion of HPLC within these courses will provide our students with an important experience in using current methodologies. Such skills are commonly required and are greatly beneficial for ensuring their competitiveness for post-graduate studies, research jobs or careers.