Role of a Juvenile Hormone Receptor in Insect Metamorphosis

林恩·瑞迪福德 建议研究阐明特异性结合保幼激素（jp 29）的核29 kDa蛋白在调节幼虫蜕皮和变态中的作用。 烟草天蛾的研究将集中在三个方面：1）分离jp 29可能与之相互作用的其他核蛋白，并随后对其进行克隆和测序以阐明其功能; 2）细胞核内的免疫细胞化学定位; 3）jp 29的发育和内分泌调节。 我们还计划从果蝇中分离含有热休克启动子控制下的Manduca jp 29 cDNA的同源基因，将用于确定该蛋白质在存在和不存在JH的情况下对发育、繁殖和JH酯酶产生的过表达的影响。 此外，免疫细胞化学研究将集中在其在各种组织中的发育和内分泌调节。 这两种昆虫的研究应有助于阐明JH在指导变态中的分子作用模式，以及Jp 29是否在成虫对JH的反应中具有功能性作用。 ***

Lynn Riddiford #IBN 9419957 Proposed are studies to elucidate the role of the nuclear 29kDa protein that specifically binds juvenile hormone (jp29) in the regulation of larval molting and metamorphosis. Studies on the tobacco hornworm, Manduca sexta, will concentrate on three aspects: 1) isolation of other nuclear protein(s) with which jp29 may interact and their subsequent cloning and sequencing to elucidate their function, 2) immunocytochemcial localization within the nucleus, and 3) developmental and endocrine regulation of jp29. We also plan to isolate the homologous gene from Drosophila containing the Manduca jp29 cDNA under the control of a heat shock promoter will be used to determine the effects of over-expression of this protein on development, reproduction, and JH esterase production in the presence and absence of JH. Also, immunocytochemical studies will concentrate on its developmental and endocrine regulation in various tissues. This study in the two insects should help elucidate the molecular mode of action of JH in directing metamorphosis and whether jp29 has a functional role in adult responses to JH. ***