Endocrine Regulation of the Ecdysteroid Cascade During Insect Metamorphosis

9514187里迪福德蜕皮类固醇可引起昆虫蜕皮和变态，幼虫生活中存在保幼激素，以允许生长和蜕皮并防止变态。干扰这些激素的作用是控制害虫的一种方法。Riddiford博士发现，在烟草角虫的蜕皮和变态过程中，蜕皮激素受体亚型及其伴侣USP存在着动态和复杂的变化模式。她现在计划研究这些受体的激素和时空调节，以及它们在细胞水平上诱导的初始转录因子，主要使用体内和体外暴露于这些激素的组织的免疫细胞化学和原位杂交分析。这些研究将提供更深入的见解，了解蜕皮类固醇如何启动和协调细胞反应，导致在蜕皮和细胞命运转换期间形成新的角质层，这是变态所必需的。通过研究保幼激素对这些细胞动力学的影响，她将为其在防止细胞命运转换方面的作用模式提供新的线索。***

9514187 Riddiford Ecdysteroids cause insect molting and metamorphosis, and juvenile hormone is present during larval life to allow growth and molting and to prevent metamorphosis. Interference with the action of these hormones is one way to control insect pests. Dr. Riddiford has found that there is a dynamic and complex pattern of changing ecdysteroid receptor isoforms and its partner USP during a molt and at metamorphosis in the epidermis of the tobacco hornworm, Manduca sexta. She now plans to study both the hormonal and the spatiotemporal regulation of these receptors and the initial transcription factors that they induce at the cellular level using primarily immunocytochemical and in situ hybridization analysis of tissue exposed to these hormones both in vivo and in vitro. These studies will provide deeper insight into how the ecdysteroids initiate and coordinate the cellular responses that lead to the formation of a new cuticle during a molt and during the switch in cell fate that is essential for metamorphosis. By studying the influence of juvenile hormone on these cellular dynamics, she will shed new light on its mode of action in the prevention of the switch of cell fates. ***