Microscopy in the Chemical Senses

该小组提案寻求资金购买三种主要设备：低温恒温器、复合研究级显微镜和共聚焦显微镜。这些项目将被安置在莫奈尔化学感官中心的组织学设施中，并将在整个中心范围内使用。该设备是由八个主要用户要求的研究项目，旨在更全面地了解化学感官。这些项目利用标准组织学、凝集素和免疫组织化学、原位杂交和原位PCR，重点研究从外周感觉受体细胞中蛋白质和核苷酸的细胞内定位到大型器官系统（如大脑和肝脏）内的代谢活动等一系列问题。

This group proposal seeks funds to purchase three major items of equipment: a cryostat, a compound, research-grade microscope and a confocal microscope. These items will be housed in the Histological Facility at the Monell Chemical Senses Center and will be utilized Center-wide. The equipment is requested by eight major users for research programs directed at a fuller understanding of the chemical senses. The projects utilize standard histology, lectin and immuno-histochemistry, in situ hybridization and in situ PCR, and focus on a range of issues from intracellular localization of proteins and neucleotides in peripheral sensory receptor cells to metabolic activity within large organ systems, e.g. the brain and the liver.