Virus Expression in Plants

9421002 Howell这个项目的目标是确定在花椰菜花叶病毒(CaMV)移动过程中宿主起作用的基因，并在这个过程中找到赋予病毒抗性的植物基因。提出了两种方法。1)对拟南芥的抗病突变体进行分离和鉴定，2)筛选出对缺陷植物病毒敏感的拟南芥抑制突变体。第二种方法是新颖的，因为它允许对抑制任何选定病毒基因或给定基因的任何特定功能突变的敏感植物进行正向选择。如果成功，它将为选择在植物抗病中重要的基因开辟新的途径。这项建议描述了一种新的方法来研究植物与病毒的相互作用和鉴定参与植物病毒复制和移动的寄主功能。将选择易受CaMV缺陷感染的拟南芥植株。由于宿主和病毒之间的相互作用在病毒运动中最为明显，因此将产生使CaMV运动蛋白失效的突变。此外，将选择抗病毒突变体，并将表征一种已被证明阻止病毒长距离传播的抗病毒特性，显然是单基因特性。***

9421002 Howell The goal of this project is to identify genes responsible for the role of the host in the movement of cauliflower mosaic virus (CaMV) and in the process to find plant genes that confer virus resistance. Two approaches are proposed. 1)Virus resistance mutants of Arabidopsis will be isolated and characterized and 2)Arabidopsis suppresser mutants susceptible to defective plant viruses will be selected. The second approach is novel in that it allows positive selection for susceptible plants that suppress mutations in any chosen viral gene or in any particular function of a given gene. If successful, it will open new avenues for the selection of genes important in plant disease resistance. %%% This proposal describes a novel approach to the study of plant-virus interactions and the identification of host functions involved in virus replication and movement in plants. Arabidopsis plants that are susceptible to infection by defective CaMV will be selected. Because the interplay between host and virus is most apparent in virus movement, mutations that disable the CaMV movement protein will be created. In addition, virus resistant mutants will be selected and a virus-resistant trait, apparently a one-gene trait, which has been shown to block long-distance virus movement will be characterized. ***