Biophysical Studies of Metalloenzymes

9507061霍夫曼多频高级顺磁共振（amr）光谱学将用于表征多金属酶的催化中心，作为涉及生物化学家，分子生物学家和微生物学家的多学科努力的一部分。 本研究将利用连续波和脉冲电子-核双共振（endor）技术以及电子自旋回波包络调制（seem）技术在X和Q波段上对蛋白质系统进行研究，重点研究三个方面：（1）双核非血红素铁中心和整数自旋非克拉默（NK）二重态的amr（nk-amr）;（二）固氮酶MoFe蛋白;（三）来自超嗜热菌的酶。 % AMR方法提供了有关金属酶催化位点的详细分析、结构和电子信息，因此在表征其功能机制方面发挥着至关重要的作用。 当然，获得这些信息是必要的，因为金属酶在生物过程中起着关键作用。 然而，由于这种酶在工业和生物技术中的重要性日益增加，这种努力正变得越来越重要（例如，腈水合酶，一种非血红素Fe酶用于将丙烯腈转化为丙烯酰胺），农业（固氮酶是在自然界中固定氮的酶），以及有毒废物的生物修复（例如，加氧酶）。 ***

9507061 Hoffman Multifrequency advanced paramagnetic resonance (amr) spectroscopy will be used to characterize the catalytic centers of multi- metallic enzymes as part of a multidisciplinary effort that involves biochemists, molecular biologists, and microbiologists. This research, which will utilize a unique capacity to employ CW and pulsed electron-nuclear double resonance (endor)and electrol spin-echo envelope modulation (eseem) techniques at both X and Q Bands, involves a number of protein systems, emphasizing three areas: (I) Binuclear non-heme iron centers and amr of non-Kramers (NK) doublets of integer-spin systems (nk-amr); (II) The nitrogenase MoFe protein; (III) Enzymes from hyperthermophiles. %%% AMR methods provide detailed analytical, structural, and electronic information about the catalytic sites of metalloenzymes, and thus play a vital role in characterizing their mechanisms of function. It is of course necessary to obtain this information because metalloenzymes play key roles in biological processes. However, this effort is acquiring increasing significance because of the growing importance of such enzymes in industry and biotechnology (eg., nitrile hydratase, a nonheme Fe enzyme is used to convert acrylonitrile to acrylamide), in agriculture (nitrogenase is the enzyme that fixes nitrogen in nature), and in efforts at bioremediation of toxic wastes (eg., oxygenases). ***