Molecular Analysis of the Brome Mosaic Virus RNA-dependent RNA Polymerase

具有（+）链rna基因组的花胶病毒构成了一个多样化的群体，包括具有重要经济意义的动物和植物病原体。这些RNA基因组的复制机制尚不清楚，宿主蛋白发挥的作用才刚刚开始确定。本研究的总体目标是了解模型（+）链RNA病毒，雀麦花叶病毒（BMV）复制其基因组的机制。实验将集中在两个关键领域，识别和表征参与病毒RNA复制的宿主因子，以及病毒蛋白在RNA依赖的RNA聚合酶中的相互作用。预期的结果很重要，因为它们将阐明病毒RNA复制的基本方面，包括宿主蛋白的作用和病毒蛋白的相互作用。许多（如果不是全部的话）这些机制细节应该在具有（+）链RNA基因组的病毒病原体中是共同的。我们对RNA复制的理解的进步将为开发抑制病毒感染的新策略提供基础。具有（+）链rna基因组的病毒构成了一个多样化的群体，包括具有重要经济意义的动物和植物病原体。这些RNA基因组的复制机制尚不清楚，宿主蛋白发挥的作用才刚刚开始确定。本研究的总体目标是了解模型（+）链RNA病毒，雀麦花叶病毒（BMV）复制其基因组的机制。预期的结果很重要，因为它们将阐明病毒RNA复制的基本方面，包括宿主蛋白的作用和病毒蛋白的相互作用。许多（如果不是全部的话）这些机制细节应该在具有（+）链RNA基因组的病毒病原体中是共同的。我们对RNA复制的理解的进步将为开发抑制病毒感染的新策略提供基础。＊＊＊

9507344 Kao Viruses with genomes of (+)-stranded RNAs make up a diverse group that include economically important pathogens of animals and plants. The mechanism of replication of these RNA genomes is not understood well, and what roles host proteins play are just beginning to be determined. The overall goal of this research is to understand the mechanism by which the model (+)-strand RNA virus, brome mosaic virus (BMV), replicates its genome. Experiments will focus on two key areas, the identification and characterization of host factors involved in viral RNA replication, and the interaction of viral proteins within the RNA-dependent RNA polymerase. The anticipated results are important because they will elucidate fundamental aspects of viral RNA replication, including the role of host proteins and the interaction of viral proteins. Many, if not all, of these mechanistic details should prove to be common among viral pathogens with (+)-strand RNA genomes. Advances in our understanding of RNA replication will provide the basis for the development of new strategies for the inhibition of viral infection. %%% Viruses with genomes of (+)-stranded RNAs make up a diverse group that include economically important pathogens of animals and plants. The mechanism of replication of these RNA genomes is not understood well, and what roles host proteins play are just beginning to be determined. The overall goal of this research is to understand the mechanism by which the model (+)-strand RNA virus, brome mosaic virus (BMV), replicates its genome. The anticipated results are important because they will elucidate fundamental aspects of viral RNA replication, including the role of host proteins and the interaction of viral proteins. Many, if not all, of these mechanistic details should prove to be common among viral pathogens with (+)-strand RNA genomes. Advances in our understanding of RNA replication will provide the basis for the development of new strategies for the inhibition of viral infection. ***