Acquisition of Instrumentation for an Oklahoma Statewide Shared NMR Facility

俄克拉荷马州立大学化学系要求购买一台600兆赫的核磁共振光谱仪，用于升级一台400兆赫的现场仪器，以及为将俄克拉荷马州各机构的学生和研究人员与核磁共振设备连接起来的数据站提供支持。该设施在全州范围内使用的关键是通过俄克拉何马ONENET进行通信，这是一个45 Mb/秒的以太网系统，连接俄克拉何马州立大学（斯蒂尔沃特）、俄克拉何马健康科学中心（俄克拉何马城）和俄克拉何马大学（诺曼）。研究型大学的数据站将用于600兆赫光谱仪的远程控制，600兆赫仪器多维光谱的离线数据分析，以及现代核磁共振波谱学研究人员和研究生的非现场指导。俄克拉何马州ONENET拥有1 Mb/秒的服务，将核磁共振设施与6所地区大学的数据站连接起来，用于现代核磁共振波谱学本科科学专业的教育，并用于分析400 MHz仪器的光谱。1993年在俄勒冈州立大学安装的用于研究固态材料的最先进的300兆赫核磁共振光谱仪也将成为全州核磁共振设施的一部分。该设施的核心是一台600 MHz核磁共振光谱仪，供俄克拉何马州健康科学中心（OHSC）、俄克拉何马大学（OU）和俄克拉何马州立大学（OSU）的研究人员用于研究糖肽、与蛋白质结合的泛醌衍生物、顺磁性蛋白质结构、合成聚合物的微观结构、原生植物细胞壁成分和海洋来源的有机天然产物。在本研究中，需要使用高频率的光谱仪来分散低频重叠的光谱信号。拟议的3通道600 MHz光谱仪将配备脉冲场梯度（PFG）和波形生成能力，用于最先进的核磁共振实验，探针范围从用于研究亚微摩尔天然材料的微探针到用于研究有限溶解度蛋白质的10毫米探针。俄勒冈州立大学的400 MHz核磁共振光谱仪于1988年安装，将升级为3通道装置，并具有与600 MHz装置相同的许多功能：脉冲场梯度，波形生成，直接和间接探针。它将用于不需要600兆赫光谱仪的实验，以及可能需要600兆赫光谱仪的某些样品的初步实验。该设施的政策将由一个全州范围的用户委员会制定，并由俄勒冈州立大学教务主任管理。设备的日常操作将由设备经理负责，他是一名全职博士光谱学家，负责在实验的设计、执行和解释方面协助和培训用户。一名全职的电子技术人员将对设备进行维护。俄勒冈州立大学的设施光谱仪通过俄克拉何马ONENET与俄勒冈州立大学和OHSC的研究中心以及俄克拉何马州的地区大学建立联系，将提供核磁共振设施和非现场用户之间的快速通信，以及设施经理和俄勒冈州立大学教师对各级学生进行现代核磁共振光谱学指导的机制。俄勒冈州立大学在通过电信提供教学方面处于领先地位。因此，该项目将显著提高三所研究型大学测定天然和合成大分子结构的研究能力，并将现代核磁共振波谱学的教育扩大到俄克拉荷马州85%以上的化学和生物化学专业本科生。俄克拉荷马州高等教育董事办公室设想，拟议中的俄克拉荷马州全州共享核磁共振设施将发展成为一个分布设施，在俄克拉荷马州的各个地点都有主要的核磁共振仪器，所有这些仪器都通过互联网与全州的科学家连接。

Support is requested for the purchase of a 600 MHz NMR spectrometer to be housed in the Department of Chemistry at Oklahoma State University, for an upgrade of an on-site 400 MHz instrument, and for data stations to link students and researchers at institutions all over the State of Oklahoma to the NMR Facility. The key to statewide use of the facility is communication via the Oklahoma ONENET, a 45 Mb/sec Ethernet system that links Oklahoma State University (Stillwater), the Oklahoma Health Sciences Center (Oklahoma City), and the University of Oklahoma (Norman). The data stations at the research universities will be used for remote control of the 600 MHz spectrometer, off-line data analysis of multidimensional spectra from the 600 MHz instrument, and off-site instruction of research associates and graduate students in modern NMR spectroscopy. The Oklahoma ONENET has 1 Mb/sec service to link the NMR facility to data stations at 6 regional universities for the education of undergraduate science majors in modern NMR spectroscopy and for analysis of spectra from the 400 MHz instrument. A state-of-the-art 300 MHz NMR spectrometer dedicated to the study of solid state materials and installed at OSU in 1993 will also become part of the statewide NMR Facility. The centerpiece of the proposed Facility is a 600 MHz NMR spectrometer to be used by researchers at the Oklahoma Health Sciences Center (OHSC), University of Oklahoma (OU), and Oklahoma State University (OSU) for the study of glycopeptides, derivatives of ubiquinone bound to proteins, structures of paramagnetic proteins, microstructures of synthetic polymers, components of primary plant cell walls, and organic natural products from marine sources. For this research, high spectrometer frequency is needed to disperse spectral signals that overlap at lower frequencies. The proposed 3-channel 600 MHz spectrometer will be equipped with pulsed field gradient (PFG) and waveform generation capabilities required for state-of -the-art NMR experiments, and with probes ranging from a microprobe for study of submicromole amounts of natural materials to a 10 mm probe with an enhanced shim set for study of proteins of limited solubility. The 400 MHz NMR spectrometer at OSU, which was installed in 1988, will be upgraded to a 3-channel unit and have many of the same capabilities as the 600 MHz unit: pulsed field gradients, waveform generation, direct and indirect probes. It will be used for experiments that do not require the 600 MHz spectrometer and for preliminary experiments on certain samples that may later require the 600 MHz spectrometer. Policies of the Facility will be established by a statewide committee of users and administered by an OSU Faculty Director. Daily operation of the Facility will be the respmnsibility of the Facility Manager, a full time Ph.D. spectroscopist with responsibilities to assist and train users in the design, execution, and interpretation of experiments. An electronics technician dedicated full time to the Facility will maintain the equipment. Linkage of the Facility spectrometers at OSU to the research centers at OU and OHSC and to the regional universities in Oklahoma via the Oklahoma ONENET will provide both rapid communication between the NMR facility and off-site users and the mechanism for instruction of students at all levels by the Facility Manager and OSU faculty in modern NMR spectroscopy. OSU is a leader in providing instruction via telecommunication. Thus the project will both markedly enhance research capabilities for determination of structures of natural and synthetic macromolecules at three research universities, and expand education in modern NMR spectroscopy to more than 85% of the undergraduate students majoring in chemistry and biochemistry in Oklahoma. The office of the Oklahoma State Regents for Higher Education envisions the proposed Olclahoma Statewide Shared NMR Facility evolving into a distributive facility with major NMR inst ruments at various sites in Oklahoma all connected by Internet to scientists throughout the state.