Stimulus-Secretion Coupling in Mucosal Mast Cells: Studies at the Single-Cell Level

摘要9513864 Fewtrell这个提议是为了研究钙在非兴奋细胞中刺激-反应偶联中的作用。已经发现钙信号的幅度和钙振荡的频率都很重要，另外的信号如蛋白激酶C也很重要。在这个提议中，将使用粘膜肥大细胞系在单细胞中研究细胞内钙和分泌变化之间的时间关系。将通过indo-1光度法测量细胞内钙，并通过恒电位安培法在同一细胞中同时测量单个含胡萝卜素颗粒的分泌。这将决定与分泌爆发相关的钙反应。荧光成像将用于同时监测多个细胞的钙反应。这些钙反应将在对许多不同刺激的反应中进行测量。本项目将研究钙在肥大细胞对特定效应物的分泌反应中的作用，这是一种刺激反应偶联，发生在许多生理反应中。***

Abstract 9513864 Fewtrell This proposal is to study the role of calcium in stimulus-response coupling in non-excitable cells. Both the amplitude of the calcium signal and its frequency of calcium oscillations have been found to be important, as well as additional signals like protein kinase C. In this proposal the temporal relation between changes in intracellular calcium and secretion will be studied in single cells using a mucosal mast cell line. Intracellular calcium will be measured by indo-1 photometry, and the secretion of individual serotonin-containing granules will be measured simultaneously in the same cell by constant potential amperometry. This will determine the calcium response associated with a burst of secretion. Fluorescence imaging will be used to monitor the calcium responses of a number of cells simultaneously. These calcium responses will be measured in response to a number of different stimuli. %%% This project will examine the role of calcium in the secretory response of mast cells to specific effectors, which is a type of stimulus response coupling, that occurs in many physiological responses. ***