Developing Adsorptive Protein Separations

Cts-9522528威斯康星麦迪逊大学的Ed LightFoot Thatcher Root摘要该研究项目的重点是蛋白质色谱(吸附)分离的关键参数的测量。核磁共振将被用来获得颗粒内扩散系数，并建立吸附柱性能的预测关联式。我们将使用磁共振成像(MRI)来研究颗粒尺度(连接弥散)和宏观水平(沟道)上的非均匀流动。研究人员还将使用核磁共振检查高柱负荷下热力学和传输的相互作用。预期的好处包括加强质量控制、降低蛋白质制造成本以及缩短从药物发现到临床应用的开发时间。这些好处将主要来自改进使用层析进行生物分离的设计程序。

CTS-9522528 Ed Lightfoot Thatcher Root University of Wisconsin Madison ABSTRACT The research project focuses on the measurement of key parameters for chromatographic (adsorptive) separation of proteins. NMR will be used to obtain intraparticle diffusion coefficients and develop predictive correlations for the performance of the adsorption column. Non-unifopm flow, both on a scale of particle dimensions (connective dispersion) and at a macroscopic level (channeling), will be studied using magnetic resonance imaging (MRI). The investigators will also examine the interaction of thermodynamics and transport at high column loadings using MRI. Anticipated benefits include enhanced quality control, lower costs of manufacturing proteins and shorter development times from drug discovery to clinical application. These benefits will result primarily from improved design procedures for bioseparations using chromatography.