Effect of Erythrocytes on Cellular Interactions Under Flow

9529673血液中循环细胞与血管壁的结合是许多生理过程中的关键步骤，例如炎症、伤口愈合、癌症转移等。以往的研究忽略了红细胞(红细胞)对这些循环细胞与血管内皮细胞黏附的影响，红细胞是血液的主要成分(体积分数高达40%)。需要回答的三个关键问题是：红细胞对细胞“滚动”的速率以及淋巴细胞和癌细胞与内皮细胞单层的结合有什么影响？观察到的影响是生化的和/或物理的吗？能否开发一个理论框架来模拟RBC增强的黏附？主要的假设是，红细胞将增强细胞与内皮细胞衬里的粘附性。这一假设将在层流室中得到验证。研究人员已经熟悉了试验室和指定的细胞。***

9529673 Jain The binding of circulating cells in the blood to the vascular wall is a key step in many physiological processes, e.g., inflammation, wound healing, cancer metastasis, etc. Previous studies have neglected the impact of red blood cells (RBC's), a major blood component (up to 40% by volume) on the adhesion of these circulating cells to the endothelial vascular lining. Three key questions to be answered are: What effect do the RBC's have on the rates of cell "rolling" and binding of lymphocytes and cancer cells to endothelial cell monolayers? Are the observed effects biochemical and/or physical in nature? Can a theoretical framework be developed to model the RBC-enhanced adhesion? The main hypothesis is that the RBC's will enhance the adhesion of the cells to the endothelial lining. The hypothesis will be tested in a laminar flow chamber. The researchers are already familiar with the test chamber and the specified cells. ***