Analysis of Molecular Interactions Influencing Turnip Crinkle Virus Pathogenesis on Arabidopsis Thaliana

Wobbe芜菁皱缩病毒（Wobbe Turnip crinkle virus）是一种植物病原体，已被证明需要两种小的蛋白质用于植物中的运动和系统感染。蛋白质的表征将继续通过定点诱变结合此类突变体的功能表征进行。缺失分析将用于确定核酸结合和协同作用所必需的蛋白质区域。将尝试分离这两个功能域。还将继续研究TCV突变体，该突变体在某些植物中不诱导过敏反应，尽管它像野生型一样复制和传播。 %植物病毒是定义最明确的植物病原体之一;它们的基因组很小，很容易进行遗传操作。它们在系统感染过程中如何在植物中移动的问题很重要，这项工作应该增加我们对这一过程的了解。 ***

9531745 Wobbe Turnip crinkle virus, a plant pathogen, has been shown to require two small proteins for movement and systemic infection in plants. Characterization of the proteins will be continued by site-directed mutagensis coupled with functional characterization of such mutants. Deletion analysis will be used to determine regions of the protein which are necessary for nucleic acid binding and cooperatively. An attempt will be made to separate the two functional domains. Work will also continue with the TCV mutant which does not induce the hypersensitive response in certain plants although it replicates and spreads as does wildtype. %%% Plant viruses are among the best defined plant pathogens; they have small genomes and are easily genetically manipulated. The question of how they move through the plant in the course of systemic infection is important to understand and this work should add to our knowledge of that process. ***