Bioanalytical Separation Equipment for the Undergraduate Laboratories

该项目资助了一个高效液相色谱（HPLC）装置和一个毛细管电泳（CE）系统。 这些生物分析分离仪器被用于以下本科实验室：生物化学和环境分析，仪器技术，生物化学实验室和独立研究。 最初，学生们在生化和环境分析实验室接触这些仪器，在那里他们对尿液中的代谢重要化合物或饮料中的人工甜味剂和添加剂进行HPLC分析。 学生提供自己的样本进行分析。 一个简单的实验CE被引入到证明使用涂层毛细管蛋白质分离的权力。 由溶菌酶、核糖核酸酶A、细胞色素C和肌红蛋白组成的蛋白质混合物可以用自由区毛细管电泳容易地分离。 在仪器技术中，学生学习优化参数和条件，以实现这些仪器上分离的高分辨率。 在生物化学实验室，学生通过蛋白质的项目研究被引入实验生物化学领域。 HPLC和CE的可用性极大地影响了蛋白质制备和分离蛋白质纯度分析的可用方法。 由于时间变得正式课程之外可用，该仪器也可以由学生做化学专业所需的各种独立的研究项目使用。

This project funds a high-performance liquid chromatography (HPLC) apparatus and a capillary electrophoresis (CE) system. These bioanalytical separation instruments are being used in the following undergraduate laboratories: Biochemical and Environmental Analysis, Instrumental Techniques, Biochemistry Lab and Independent Study. Initially students are exposed to these instruments in the Biochemical and Environmental Analysis Lab, where they carry out HPLC analysis of metabolically important compounds in the urine or artificial sweeteners and additives in beverages. Students provide their own samples for the analysis. A simple experiment on CE is being introduced to demonstrate the power of protein separation using a coated capillary. A protein mixture consisting of lysozyme, ribonuclease A, cytochrome C, and myoglobin may be readily separated using free-zone capillary electrophoresis. In Instrumental Techniques, the students learn to optimize parameters and conditions to achieve high resolution for separations on these instruments. In Biochemistry Lab, students are introduced to the field of experimental biochemistry through a project study of a protein. The availability of HPLC and CE greatly affects the methods available for the preparation of the protein and the analysis of the purity of the isolated protein. As time becomes available outside of the formal courses, the instrument can also be used by students doing various independent research projects which are required of chemistry majors.