CAREER: Identification of the Site-of-Action in Wheat of Pyrenophora tritici-repentis Toxin

9600914 Ciuffetti Research：这是一个职业奖。现代植物生物学的一个主要目标是对调节植物与微生物相互作用的因子进行分子定义。PI正在调查一种被称为小麦褐斑病的疾病，这种疾病是由真菌引起的，Pyrenophora tritrii -repentis。这种真菌是致病性的，因为它产生宿主选择性毒素（HST），称为Ptr毒素。与所有其他hst不同，Ptr毒素是一种蛋白质，是单个基因的直接产物。因此，这种真菌的致病性是由一个产生毒素的基因调控的。此外，对Ptr毒素敏感性的遗传评价表明，小麦对毒素的反应以及对病原体的敏感性由单个显性基因控制。该奖项的研究部分由四个具体目标组成，其中包括多种方法，包括免疫学、生化和遗传学方法，以确定Ptr毒素的作用部位：目标1。对PTR毒素敏感的二倍体小麦的鉴定与遗传特性研究。目标2。PTR毒素标记衍生物的生产和作用位点/受体的生化鉴定。目标3。利用酵母双杂交系统对毒素与其作用位点/受体相互作用进行遗传分析。目标4。PTR毒素作用位点/受体的免疫细胞化学定位。教育：要想在植物科学领域有一个有效的学术生涯，就需要将研究和教育结合起来。俄勒冈州立大学和植物学和植物病理学系致力于这一整合。为了与大学和系里培养研究生和本科生的使命保持一致，招募代表性不足的个人进入科学领域，并向社区推广，教育职业规划由四个主要目标组成：目标1。影像显微镜、计算机增强成像和信息技术在植物科学本科教学中的应用。目标2。培养研究生成为更有效的植物科学教育者。目标3。对妇女的承诺和对本科生从事科学事业的培训。目标4。服务社会。该PI的研究项目为教育活动提供了基础的科学主题。

9600914 Ciuffetti Research: This is a CAREER Award. A major goal of modern plant biology is a molecular definition of factors that regulate plant-microbe interactions. The PI is investigating a disease called tan spot of wheat which is caused by the fungus, Pyrenophora tritici-repentis. This fungus is pathogenic because it produces the host-selective toxin (HST) called Ptr toxin. Unlike all other HSTs, Ptr toxin is a protein and the direct product of a single gene. Thus, pathogenicity in this fungus is regulated by a single gene for toxin production. Also, genetic evaluation of sensitivity to Ptr toxin has demonstrated that the wheat response to the toxin and consequently, susceptibility to the pathogen, is controlled by a single dominant gene. The research component of this award is composed of four specific objectives which encompass a diversity of approaches including an immunological, biochemical, and genetical approach to the identificaiton of the site-of-action of Ptr toxin: Objective 1. IDENTIFICATION AND GENETIC CHARACTERIZATION OF DIPLOID WHEATS SENSITIVE TO PTR TOXIN. Objective 2. PRODUCTION OF LABELED DERIVATIVES AND BIOCHEMICAL IDENTIFICATION OF THE SITE-OF- ACTION/RECEPTOR OF PTR TOXIN. Objective 3. GENETICAL ANALYSIS OF INTERACTIONS BETWEEN THE TOXIN AND ITS SITE-OF-ACTION/RECEPTOR USING THE YEAST TWO-HYBRID SYSTEM. Objective 4. IMMUNOCYTOCHEMICAL LOCALIZATION OF THE SITE-OF- ACTION/RECEPTOR OF PTR TOXIN. Education: Development of an effective academic career in the plant sciences requires integration of both research and education. Oregon State University and the Department of Botany and Plant Pathology are committed to this integration. In keeping with the mission of the University and the Department toward graduate and undergraduate training, the recruitment of underrepresented individuals into science, and outreach to the community, the education career plan is composed of four main objectives: Objective 1. INCORPORATION OF VIDEOM ICROSCOPY, COMPUTER ENHANCED IMAGING AND INFORMATION TECHNOLOGIES IN TEACHING UNDERGRADUATE PLANT SCIENCES. Objective 2. TRAINING GRADUATE STUDENTS TO BE MORE EFFECTIVE EDUCATORS IN THE PLANT SCIENCES. Objective 3. COMMITMENT TO WOMEN AND TRAINING OF UNDERGRADUATES FOR A CAREER IN THE SCIENCES. Objective 4. SERVICE TO THE COMMUNITY. This PI's research projects provide the underlying scientific subject for the education activities.