Acquisition and Development of High Resolution Digital Imaging Facility for the Investigation of Cytoskeletal and Membrane Protein Molecular Dynamics

要求项目摘要仪器支持耶鲁大学细胞动力和细胞骨架小组四个实验室的互动研究工作。这项提议旨在补充正在进行的合作研究工作，并扩大我们在分子动力学和共聚焦激光扫描显微镜领域的技术能力。具体地说，我们已经申请了资金，用于购买最先进的共焦激光扫描显微镜，以及设计和建造用于研究分子动力学的高分辨率数字成像系统所需的部件。该系统将实现：荧光光漂白和光激活分析、单光束梯度激光捕获、细胞烧蚀和荧光探针的实时定量微光成像。该奖项推动的研究活动将包括神经元生长和目标识别的分子动力学研究(Forscher)；肌动蛋白和基于微管的分子马达的分子和功能分析(Mooseker、Wolenski和Rosenbaum)。所要求的仪器还将大大加强生物系成像培训和研究设施的研究和培训任务。这包括本科生和研究生的光学方法及其在分子细胞生物学中的使用方面的高级课程。

Project Summary Instrumentation is requested to support the interactive research efforts of the four laboratories comprising the Yale Cell Motility and Cytoskeletal Group. This proposal is aimed at complementing ongoing collaborative research efforts and extending our technical capabilities in the area of molecular dynamics and confocal laser scanning microscopy. Specifically, we have requested funding for acquisition of a state-of-the-art confocal laser scanning microscope and requisite components for design and construction of a high resolution digital imaging system for studying molecular dynamics. This system will implement: fluorescence photobleaching and photoactivation analyses, single beam gradient laser trapping, cell ablation and quantitative low light level imaging of fluorescent probes in real time. Research activities facilitated by this award will include investigation of the molecular dynamics of neuronal growth and target recognition (Forscher); molecular and functional analyses of actin and microtubule-based molecular motors (Mooseker, Wolenski and Rosenbaum). The requested instrumentation will also significantly augment the research and training mission of the Department of Biology's Training and Research Facility for Imaging. This includes advanced courses in optical methods and their use in molecular cell biology at both undergraduate and graduate levels.