A High Speed Centrifuge for Preparation of Biological Materials

请拨经费购买一台落地式冷冻高速离心机和四个转子。该离心机将取代目前由位于什里夫波特的路易斯安那州立大学拥有的一台已有16年历史的贝克曼J2-21离心机。生物科学系、化学系和物理系的6名教职员工需要一台高速离心机进行蛋白质、核酸和细胞器的常规制备，用于研究实验。离心机也是为研究生和本科生的研究和实验课程准备材料所必需的。具体要求的仪器是贝克曼阿凡提J-25I高性能离心机。需要四个转子来允许必要的离心程序：一个大容量转子（容纳六个500毫升的瓶子），一个高速转子50毫升管（75,600 x g），一个低速摆动桶转子，可容纳可互换的桶和适配器，用于多种用途，一个摆动桶转子能够达到26,500 x g。

Funding is requested for the purchase of a floor-model, refrigerated high speed centrifuge and four rotors. The centrifuge would replace the one preparative centrifuge currently owned by Louisiana State University at Shreveport, a sixteen-year old Beckman J2-21 centrifuge. A high speed centrifuge is required for the routine preparation of proteins, nucleic acids and organelles for research experiments by six faculty members in the Departments of Biological Sciences and Chemistry and Physics. The centrifuge is also necessary for the preparation of materials for graduate and undergraduate student research and for laboratory courses. The specific instrument requested is the Beckman Avanti J-25I High Performance Centrifuge. Four rotors are desired to permit necessary centrifugation procedures: a large volume rotor (to hold six 500 ml bottles), a high speed rotor for 50 ml tubes (to 75,600 x g), a low-speed swinging bucket rotor that accommodates interchangeable buckets and adapters for multiple purposes and a swinging bucket rotor capable of forces to 26,500 x g.