A Biochemical Genetic Analysis of Inositol Metabolism in Plastids

约翰逊9604527 本课题研究肌醇在植物中的合成。 虽然对动物中的肌醇磷酸信号传导了解很多，但植物中的相应问题尚未得到很好的研究。 这项工作的总体长期目标是了解肌醇在亚细胞器生物学中的作用。 PI在以前的研究中已经表明，关键的生物合成酶，肌醇-1-磷酸合酶，具有时间和空间控制的异构体，这是新颖的和出乎意料的。 这个特殊的项目将涉及对肌醇和磷酸肌醇合成位点的详细研究。 免疫细胞化学分析，原位杂交研究和酶活性测量的组合将使用菜豆作为模型进行。 这项工作将有助于发展对植物细胞信号传导的理解，以及对重要代谢途径的调节。 本课题研究肌醇在植物中的合成。 虽然对动物细胞中的肌醇磷酸信号传导了解很多，但植物中的相应问题尚未得到很好的研究。 这个特殊的项目将涉及详细研究的网站肌醇和磷酸肌醇合成豆类植物。 这些结果将为植物生长、发育和分化的调控过程提供重要信息。

Johnson 9604527 This project is concerned with synthesis of inositol in plants. While much is known about inositol phosphate signaling in animals, the corresponding issues in plants have not been well studied. The overall long-term goal of the work is to understand inositol's role in the biology of subcellular organelles. The PI has shown, in previous studies, that the key biosynthetic enzyme, myo-inositol-1-phosphate synthase, has temporally and spatially controlled isoforms which is novel and unexpected. This particular project will involve a detailed study of the sites of inositol and phosphoinositol synthesis. A combination of immunocytochemical analyses, in situ hybridization studies, and enzyme activity measurements will be performed using Phaseolus vulgaris as a model. This work will help in developing an understanding of cell signaling in plants, as well as regulation of an important metabolic pathway. This project is concerned with synthesis of inositol in plants. While much is known about inositol phosphate signaling in cells of animals, the corresponding issues in plants have not been well studied. This particular project will involve a detailed study of the sites of inositol and phosphoinositol synthesis in bean plants. The results will provide important information about processes which control plant growth, development, and differentiation.