Pattern recognition receptors: discovery, function and applications in crops for durable disease control (PRR-CROP)

最近在模式植物拟南芥（Arabidopsis）中的工作导致了模式识别受体（PRRs）的发现，其识别广泛类别的病原体分子，并提供了开发广谱疾病控制的前景。我们的建议的新颖之处在于开发这种知识，以便将其应用于作物物种。我们专注于谷物、芸苔和葡萄藤，并专注于识别真菌和卵菌的PRR，这些真菌和卵菌是这些作物的主要病原体。A）鉴定和测试拟南芥和选定作物（例如大麦、小麦、番茄、葡萄藤、芸苔属（P1、P2、P3、P4和AP 1））识别的新型细菌、真菌和卵菌病原体相关分子模式（PAMP）。). B）在拟南芥和所选作物例如大麦、小麦、葡萄藤、芸苔属（P1、P2、P4和AP 1）中鉴定新的PRR。C）将拟南芥PRRs转化到作物中并评估抗性表型（P1、P2和AP 2）。我们期望将多达5种PRR转化到大麦、小麦和芸苔属中，并且将用感染每种作物物种的至少2种病原体测试它们。D）评价葡萄藤和各种谷物和芸苔属种质（P2、AP 1和AP 2）中对基础抗性应答的发育和环境影响。

Recent work in the model plant Arabidopsis has led to the discovery of pattern recognition receptors (PRRs) which recognise broad classes of pathogen molecules and offer the prospect of developing broad-spectrum disease control. The novel aspect of our proposal is to develop this knowledge so that it can be applied to crop plant species. We concentrate on cereals, brassica and grapevine, and focus on PRRs that recognise the fungi and oomycetes which are the major pathogens of these crops. The objectives of the proposal are:A) Identification and testing of novel bacterial, fungal and oomycete pathogen-associated molecular patterns (PAMPs) that are recognised by Arabidopsis and selected crops, e.g. barley, wheat, tomato, grapevine, brassica (P1, P2, P3, P4 and AP1). ). We use a variety of approaches that should lead to the discovery of 10 or more PAMPsB) Identification of new PRRs in Arabidopsis and selected crops, e.g. barley, wheat, grapevine, brassica (P1, P2, P4 and AP1). We expect that between 5 and 10 PRRs could be identified, or genetically defined within the 3-year periodC) Transformation of Arabidopsis PRRs into crops and evaluation of the resistance phenotype (P1, P2 and AP2). We expect to transform up to 5 PRRs into barley, wheat and brassica and will test them with at least 2 pathogens which infect each crop species.D) Evaluation of developmental and environmental influences on basal resistance responses in grapevine and a diverse range of cereal and brassica accessions (P2, AP1 and AP2).