CISE Research Instrumentation: Modeling and Image Analysis of Biofilm Systems

汉密尔顿，马丁A.；蒙大拿州立大学CISE研究仪器：生物膜系统的建模和图像分析仪器拨款将支持一个高性能工作站，用于生物膜过程的建模和图像分析。生物膜是附着在基质上的凝胶状物质，由微生物及其分泌的黏液组成。许多涉及液体的自然和工程系统都受到生物膜的深刻影响。生物膜的观察主要是通过精密显微镜和表面科学仪器的数字化图像。量化图像和模拟生物膜过程是很重要的。该工作站需要支持三个相互关联的复杂计算项目：分析生物膜图像，模拟生物膜中的传输现象，构建生物膜发育的三维细胞自动机模型。定量图像分析需要估计基本生物和物理化学机制的参数。许多生物膜含有通道，这些通道在细胞簇周围提供基本的循环系统。任何实际的营养物浓度分布和生物膜中相关微生物生长的模型都必须考虑通道中的平流运输和扩散。需要三维模型来表示微生物之间的相互作用、微生物与环境的相互作用以及微生物对生物膜的附着和脱离等关键过程。元胞自动机建模是构建可信的三维模型的最可行的初始策略。

CDA-96-17579 Hamilton, Martin A..Harkin, Gary J. Montana State University - Bozeman CISE Research Instrumentation: Modeling and Image Analysis of Biofilm Systems The instrumentation grant will support a high-powered workstation requested for use in modeling and image analyses of biofilm processes. A biofilm is a gelatinous mass, consisting of microbes and the slime they secrete, attached to a substratum. Many natural and engineered systems involving liquids are profoundly influenced by biofilms. Observations of biofilms are mainly via digitized images from sophisticated microscopes and surface science instruments. It is important to quantify the images and to model biofilm processes. The requested workstation is necessary to support three interrelated, complex computational projects: analyzing biofilm images, simulating transport phenomena in biofilms, and constructing a 3-dimensional cellular automata model of biofilm development. Quantitative image analysis is required to estimate parameters for fundamental biological and physico- chemical mechanisms. Many biofilms contain channels that provide a rudimentary circulatory system around cell clusters. Any realistic model of nutrient concentration profiles and associated microbial growth in the biofilm must account for advective transport in the channels, as well as diffusion. A 3-dimensional model is required to represent key processes such as interactions among microbes, interactions of microbes with the environment, and microbial attachment to and detachment from the biofilm. Cellular automata modeling is the most feasible initial strategy for constructing a plausible 3-dimensional model.