CAA: Protein Folding in Yeast/Gene Silencing in Bacteria

Lopilato 9629780为了更新Lopilato博士的研究技能，提供了这个职业发展奖。她在达纳法伯癌症研究所（波士顿，马萨诸塞州）的酵母分子生物学研究实验室休了六个月的假，随后在家里的机构减少了六个月的教学负担，以便将这些技能应用到她的实验室研究中。通过采用新的技术和方法来解决她的实验室在过去几年里一直在研究的问题，她的研究范围和有效性都将得到扩大。在一个以研究为主要活动的机构参加一个研究项目，将使她有时间集中精力提高她的研究效率和潜力。为了在细菌基因沉默方面取得进展，洛皮拉托博士将受益于接近项目的新方法。她将在休假的前六个月研究酵母中的蛋白质折叠，这显然是她研究兴趣的扩展。特别是，她将利用两种类型的遗传相互作用，即合成致死相互作用和抑制分析，来检测和鉴定酵母细胞内质网中参与蛋白质折叠的蛋白质。这些方法将在休假的后六个月直接应用到她自己的研究中，希望能找到参与基因沉默的新蛋白质。她将在研究机构获得的一项特殊技能是使用聚合酶链反应进行DNA扩增，这将立即纳入她的研究和她所教授的课程中。促进她研究的活动包括上述遗传和分子方法以及细胞生物学方法，如蛋白质标记和定位技术。所有这些方法和方法都将增强她从事研究项目的能力，并在西蒙斯学院保持一个活跃的研究实验室，这是一个教学和教师研究都是重中之重的本科学院。

Lopilato 9629780 To update the research skills of Dr. Lopilato, this Career Advancement award is provided. A six month sabbatical is being taken in a yeast Molecular Biology research laboratory at Dana Farber Cancer Institute (Boston, MA), followed by six months with a reduced teaching load at the home institution, in order to apply those skills to research in her laboratory. Both the scope and effectiveness of the her research will be expanded by employing new techniques and approaches to problems that have been under study in her laboratory for the past few years. Participating in a research project at an institution where research is the primary activity will allow her to have time to focus her energies on increasing her research productivity and potential. In order to progress in her work on gene silencing in bacteria, Dr. Lopilato will benefit from new ways of approaching projects. She will be studying protein folding in yeast during the first six months of the sabbatical, which is a clear expansion of her research interests. In particular, she will be exploiting two types of genetic interactions, namely synthetic lethal interactions and suppressor analysis, to detect and identify proteins involved in protein folding in the endoplasmic reticulum of yeast cells. These approaches will be directly applied to her own research during the second six months of the sabbatical, and hopefully, will lead to identifying new proteins which are involved in gene silencing. A specific skill that she will gain while at the research institution is DNA amplification using polymerase chain reaction, which will be incorporated immediately into her research and into the courses that she teaches. The activities that will advance her research include the genetic and molecular approaches described above and cell biology approaches such as protein tagging and localization techniques. All of these methods and approaches will enhance her ability to pursue research projects and maintain an active research l aboratory at Simmons College, an undergraduate college where both teaching and faculty research are high priorities.