Genetic Analysis of Female Gametophyte Development and Function

雌配子体是一个绝对必要的结构，它参与种子发育、花粉管引导、受精和诱导种子发育等生殖功能。 尽管其重要性，很少有人知道的分子过程控制雌配子体的发展和功能。本提案中实验的长期目标是了解这些过程。我们将从遗传学的角度来解决这个问题。该方案有四个具体目标：（1）鉴定雌配子体发育缺陷的突变体，（2）从遗传上表征雌配子体突变体，（3）确定FG突变体中受影响的发育步骤，以及（4）分离一个雌配子体基因。这些研究将导致介导雌配子体发育和功能的关键基因的分离，并应提供这些过程是如何调节的新见解。因为 生殖过程的雌配子体介导，这一基本 这些知识可以切实地应用于控制种子的可视性和活力。

The female gametophyte is an absolutely essential structure that is involved in several reproductive functions such as seed development, pollen tube guidance, fertilization, and the induction of seed development. Despite its importance, very little is known about the molecular processes controlling female gametophyte development and function. The long-term goal of the experiments in this proposal is to understand these processes. We will take a genetic approach to this problem. This proposal has four specific goals: (1) identify mutants with defects in female gametophyte development, (2) characterize the female gametophyte mutants genetically, (3) determine the developmental steps affected in the FG mutants, and (4) isolate one female gametophyte gene. These studies will lead to the isolation of key genes mediating female gametophyte development and function and should provide new insight into how these processes are regulated. Because of the reproductive processes the female gametophyte mediates, this basic knowledge can feasibly have applications to the control of seed visability and vigor.