Mechanism of Nonhomologous RNA Recombination in Brome MosaicVirus: Separating Replication and Recombination

小行星9630794 以雀麦花叶病毒（BMV）为模型系统，研究RNA病毒中RNA重组的分子机制。BMV突变体的分离和特点，在任期内的前一个奖项已经证明，复制和重组功能可以解开。作为一种方法来了解如何BMV蛋白参与非同源异源介导的重组，我们将1。分析1a解旋酶结构域在重组中的参与，2.分析RNA聚合酶（2a蛋白）核心结构域在重组中的参与; 3.研究2a蛋白N结构域在重组中的作用。 关于非同源RNA-RNA重组的机制，只有有限的信息可用。这种重组在RNA病毒基因组可塑性中起着关键作用，因为它可以导致新病毒株和物种的产生。对RNA-RNA重组的基本机制的理解将为病毒进化和多样性提供有价值的见解，并可提供可用于应用目标的重要实用信息。 ***

9630794 Bujarski The molecular mechanisms of RNA recombination in RNA viruses using brome mosaic virus(BMV) as a model system will be investigated. BMV mutants isolated and characterized during the tenure of the previous award has demonstrated that replication and recombination functions can be unlinked. As an approach to understanding how BMV proteins participate in nonhomologous heteroduplex-mediated recombination, we will 1. analyze the involvement of the 1a helicase domain in recombination, 2. analyze the involvement of RNA polymerase (2a protein) core domain in recombination and 3. study the role of the N-domain of the 2a protein in recombination. %%% Only a limited amount of information concerning the mechanisms involved in non-homologous RNA-RNA recombination is available. Such recombination plays a key role in RNA virus genome plasticity, as it can lead to the generation of new virus strains and species. An understanding of the basic mechanisms of RNA-RNA recombination will provide valuable insight into viral evolution and diversity and could provide important practical information which could be used for applied goals. ***