CAREER: New Methods of Creating Artifical Receptors for Proteins

马利克教授的研究重点是基于脂质体的蛋白质表面受体的设计、合成和评估。由含有脂质的金属、可聚合离子脂和可聚合氢键脂类衍生的混合脂质体与模板蛋白碳酸酐酶平衡，以创建蛋白质表面的三维互补复制品。合成靶标包括含有不同头部基团的脂类和可光聚合的二烯或二炔尾巴，平衡后，通过光化学聚合获得半刚性的蛋白质特异性受体。光聚合模板合成的有效性是通过测量蛋白质-复制品结合常数来量化的。教育活动包括介绍一门生物有机学研究生课程，并通过在网上建立一个虚拟有机化学教室来满足本科生的需求，以促进学生与教师的交流。通过这一职业奖项，有机动力学项目支持北达科他州大学化学系桑库·马利克教授的研究和教育活动。马利克教授的研究重点是通过使用特定的蛋白质来指导与其相互作用的一组分子的形成，从而创建一个三维蛋白质识别表面。随后将这些分子化学连接到聚合物中，有效地在所得聚合物中留下了蛋白质的足迹。与其他分子识别方法所涉及的大量合成工作不同，这种方法有望简单而快速地为目标蛋白质产生高度特异的结合位点。马利克教授的教育活动包括开发针对有机化学和生物化学之间的接口的研究生课程，以及通过网络虚拟教室促进师生交流。

Professor Mallik's research focuses on the design, synthesis and evaluation of liposome based receptors for protein surfaces. Mixed liposomes derived from metal containing lipids, polymerizable ionic lipids, and polymerizable hydrogen bonding lipids are equilibrated with the template protein carbonic anhydrase to create a three-dimensional, complementary replica of the protein surface. Synthetic targets include lipids containing differing head groups and photopolymerizable diene or diyne tails that, after equilibration, are photochemically polymerized to achieve a semi-rigid, protein-specific receptor. Efficacy of the photopolymerized template synthesis is quantified by measuring the protein-replica binding constants. Educational activities include introducing a graduate bio-organic course and addressing undergraduate needs by establishing a Virtual Organic Chemistry classroom on the Web to facilitate student-teacher communication. With this CAREER award, the Organic Dynamics Program supports the research and educational activities of Professor Sanku Mallik of the Department of Chemistry at the University of North Dakota. Professor Mallik's research is focused on the creation of a three-dimensional protein recognition surface by using a specific protein to direct the formation of a collection of molecules with which it interacts. Subsequent chemical linking of these molecules into a polymer effectively leaves a footprint of the protein in the resulting polymer. In contrast to the considerable synthetic effort involved in other approaches to molecular recognition, this approach is expected to generate simply and rapidly a highly specific binding site for the target protein. Professor Mallik's educational activities include developing a graduate curriculum targeting the interface between organic and biological chemistry and facilitating student-teacher communication through a Virtual Classroom on the Web.