Effects of Cyclic Strain and Oxidative Damage on Airway Epithelial Cell Permeability

9705600排列在肺部呼吸道的水细胞保护肺部免受吸入毒素和恶劣环境条件的影响。这种保护性屏障的维持依赖于细胞之间的接触和损伤后细胞的重新生长。臭氧、香烟烟雾或感染剂引起的氧化物种可能会损害细胞屏障。细胞对这种损伤的反应和屏障中的破坏的修复还不是很清楚。此外，尽管肺部的空气流动会引起呼吸道扩张的变化，但人们对这种机械张力对呼吸道功能的影响知之甚少。这项建议将调查氧化损伤后呼吸道细胞屏障的维护和修复。这项拟议工作的一个主要目标是开发在细胞被机械拉伸时评估屏障功能的技术。目前还没有测量拉伸细胞的阻挡性能的方法。一种新的设备将被用来对生长在弹性膜上的肺细胞施加拉伸。电池的阻挡性能将通过测量电池两端的电阻来确定。使用这个组织模型，将测量细胞对氧化损伤的反应，并将评估组织的修复。此外，还将调查在保护细胞或促进修复方面可能重要的因素。这将使人们对肺的损伤和修复有更好的了解。***

9705600 Waters Cells lining the airways of the lung protect the lung from inhaled toxins and harsh environmental conditions. Maintenance of this protective barrier depends upon the contacts between the cells and the re-growth of cells following injury. The cell barrier can be injured by oxidative species due to ozone, cigarette smoke, or infectious agents. The response of the cells to such injury and the repair of disruptions in the barrier are not well understood. Furthermore, although air flow in the lungs causes variations in the expansion of the airways, little is known about the effect of such mechanical strain on airway function. This proposal will investigate the maintenance and repair of the airway cell barrier following oxidant injury. A major goal of the proposed work is to develop techniques for the evaluation of barrier function while cells are mechanically stretched. There is currently no method for measuring the barrier properties of stretched cells. A novel device will be used to apply stretch to lung cells grown on elastic membranes. The barrier properties of the cells will be determined by measuring the electrical resistance across the cells. Using this tissue model, the response of the cells to oxidative damage will be measured, and the repair of the tissue will be evaluated. In addition, factors which may be important in protecting the cells or in enhancing repair will be investigated. This will lead to a better understanding of the injury and repair of the lung. ***