RPG: Fast Spectroscopic Measurements of the Unwinding Kinetics of DNA Hairpin Molecules

小行星9707480 这是一项针对女科学家和女工程师的研究规划补助金。 安萨里博士打算研究DNA螺旋-卷曲转换途径，并确定和表征该途径中可能的中间体沿着。 她打算通过启动解螺旋过渡和监测松弛回到平衡的动力学来做到这一点。 有一些证据表明，DNA片段和配体之间的主要相互作用位点之外的侧翼序列可以使DNA双链体更不稳定，导致与配体的反应性增加。所描述的实验将最终有助于我们理解DNA的结构，热力学和动力学特性的相互作用以及反应配体和蛋白质的识别机制。 ***

9707480 Ansari This is a Research Planning Grant for Women Scientists and Engineers. Dr. Ansari intends to study the DNA helix-coil transition pathway, and identify and characterize possible intermediates along that pathway. She intends to do this by initiating the uncoiling transition and monitoring the kinetics of relaxation back to equilibrium. There is some evidence that suggests that flanking sequences outside a primary interaction site between a section of DNA and a ligand can render the DNA duplex more unstable, resulting in an increased reactivity with the ligand. The experiments described will ultimately contribute to our understanding of the interplay of the structural, thermodynamic, and dynamic characteristics of DNA and the recognition mechanisms of reacting ligands and proteins. ***