RPG: Motion Ordered Correlation NMR Spectroscopy for Determining Multiple Protein Conformations in Solution

小行星9707550 这是一项针对女科学家和女工程师的研究规划补助金。 何博士将开发扩散或迁移率有序的同调相干相关NMR光谱学。 通过施加脉冲电场和磁场梯度，将电泳迁移率或扩散的维度引入到均匀相关光谱（COSY）中。在扩散的电泳流的维度中，不同蛋白质的COSY谱将根据其电泳迁移率或扩散速率进行排序。 因此，传统的COSY光谱的蛋白质将被分离和它们的相互作用的研究。 该技术将为研究分子信号传导过程中，DNA转录过程中或正常和异常细胞生长过程中的折叠过程和蛋白质相互作用提供新的信息。 ***

9707550 He This is a Research Planning Grant for Women Scientists and Engineers. Dr. He will develop diffusion or mobility ordered homonuclear coherence correlated NMR spectroscopy. A dimension of electrophoretic mobility or diffusion will be introduced to the homonuclear correlated spectroscopy (COSY) by applying a pulsed electric field and magnetic field gradients. In a dimension of electrophoretic flow of diffusion, COSY spectra of different proteins will be sorted according to their electrophoretic mobilities or diffusion rates. Hence, the traditional COSY spectra of the proteins will be separated and their interactions studied. The technique being developed here will provide new information for studying folding processes and protein interactions during molecular signaling processes, during DNA transcription, or during normal and abnormal cell growth. ***