Optimization and Applications of cspA, the Major E.coli Cold-Shock Promoter

9707729 Baneyx这项研究的目的是尽量减少在大肠杆菌中产生的蛋白质的不正确折叠和聚集，从而减少下游加工中非常昂贵的部分(重新折叠)。这一建议遵循了之前的观察结果，即低温、可诱导的冷休克启动子驱动重组蛋白的适当折叠。不幸的是，在较短的时间后，启动子在较低的温度下被抑制，蛋白质产量下降。这项研究的两个主要目的是消除位于冷休克启动子基因5‘非编码区的阻遏基因片段，并共表达蛋白质二硫键异构酶，以促进蛋白质的正确折叠和二硫键。***

9707729 Baneyx The purpose of this research is to minimize the improper folding and aggregation of proteins produced in E. coli, hence reducing a very costly part (refolding) of the downstream processing. This proposal follows previous observations that a low-temperature, inducible cold-shock promoter drives the proper folding of recombinant proteins. Unfortunately, after a short period the promoter is repressed at the lower temperature and protein production drops. The two major aims of the research are to eliminate the repressor gene segment believed to lie on the 5' non- coding region of the cold-shock promoter gene and to coexpress protein disulfide isomerases to promote the proper folding and disulfide bonding of the proteins. ***