Hydrodynamic Manipulation of DNA Molecules

CTS-9707778 R.G.密歇根州拉森大学 总结 将进行布朗动力学模拟来预测拉伸 长DNA分子在简单流动中的行为，包括均匀延伸， 均匀、剪切和平面Poiffille流。 这些模拟的结果将是 直接与单长，荧光，DNA分子的实验相比， 对应的简单流程。 这项研究将考虑长的聚合物分子 拉伸或剪切流，并确定是否物理力 描述稳定状态下均匀流动的理论，适用于复杂的非均匀瞬态 流程 当与实验结果结合时，模拟还可以 这有助于解释为什么光散射研究表明在这种流动中聚合物几乎没有拉伸。 这些研究不仅在聚合物流体力学的传统领域有应用， 如湍流减阻和现在通过多孔介质，但也与 涉及DNA微操作的技术，包括 “流体固定”，即，利用流体动力学流动来拉伸长DNA分子，以帮助 基因图谱

CTS-9707778 R.G. LARSON UNIV. OF MICHIGAN SUMMARY Brownian dynamics simulations will be performed to predict the stretching behavior of long DNA molecules in simple flows, including uniform extensional, uniform, shear, and plane Poiseuille flows. The results of these simulations will be compared directly with experiments on single long, fluorescing, molecules of DNA in corresponding simple flows. The research will consider the transient process by which long polymer molecules stretch out in extensional or shear flows, and determine whether or not the physical forces that describe uniform flows at steady state work for the complex unraveling transient flow processes. The simulations, when combined with the experimental results, may also help explain why light scattering studies indicate little polymer stretching in such flows. These studies have applications not only in traditional areas of polymer fluid mechanics, Such as turbulent drag reduction and now through porous media, but are also relevant to Technologies involving DNA micro-manipulation, including the new technology of "fluid fixation", i.e., the use of hydrodynamic flows to stretch long DNA molecules to aid in genetic mapping.