US-Egypt Cooperative Research: An Anchored Comparative Map of the River Buffalo Genome

描述：该奖项旨在支持德克萨斯州大学城德克萨斯农工大学兽医病理生物学系James E. Womack博士和埃及开罗国家研究中心（NRC）细胞生物学系Soheir El-Nahas博士的合作项目。这项联合研究的目的是利用埃及实验室产生的一组杂交体细胞，绘制最初用于美国实验室牛基因定位的比较锚位点。这些将包括比较锚点标记位点（CATs）引物（如适用），以及定制设计的牛PCR引物，用于锚点位点难以用CATs引物定位。目标是建立一个水牛基因组的比较图，以10厘米的间隔与牛和其他哺乳动物（包括人类和老鼠）的基因组图固定在一起。这些地图将允许从其他物种的高分辨率地图中“比较候选位置克隆”水牛的经济利益基因。范围：该研究项目将允许两位经验丰富的研究人员在一个对美国和埃及的科学利益具有重大潜力的项目上进行合作。通过鉴定具有重要经济意义的性状和标记的基因，该项目对埃及水牛的改良具有巨大潜力。美国实验室将能够继续寻找人类和牛之间的染色体同源性，因为大多数cat引物尚未在牛身上进行测试，因此没有绘制地图。因此，埃及实验室发现的任何在水牛身上起作用的引物都很有可能在牛身上起作用，然后可以添加到牛的地图上。合作筛选CATs引物的最终结果将比任何一个实验室单独进行的筛查更有效。该提案符合INT的目标，即支持美国与外国在对双方都有共同利益的领域进行合作。该项目得到了美国-埃及联合基金计划的支持，该计划向两国的科学家和工程师提供赠款，以开展这些合作活动。＊＊＊

9713618 Womack Description: This award is for support of a cooperative project by Dr. James E. Womack, Department of Veterinary Pathobiology, Texas A&M University in College Station, TX, and Dr. Soheir El-Nahas, Department of Cell Biology at the National Research Center (NRC) in Cairo, Egypt. The objective of this joint research is to utilize a panel of hybrid somatic cells, generated in the Egyptian laboratory, to map comparative anchor loci developed initially for use in the cattle gene mapping in the US laboratory. These will include comparative anchor tagged sites (CATs) primers where applicable, and custom designed bovine PCR primers for anchor loci recalcitrant to mapping with CATs primers. The goal is to build a comparative map of river buffalo genome, anchored at ten centimorgan intervals to the maps of cattle and other mammals including human and mice. These maps will permit "comparative candidate positional cloning" of genes of economic interest in buffalo from the high resolution maps of other species. Scope: This research project will allow two experienced researchers to collaborate on a project of significant potential to the scientific interests in the United States and in Egypt. The project has great potential for the improvement of buffaloes in Egypt through the identification of genes for economically important traits and markers. The US laboratory will be able to continue its pursuit of identifying the chromosomal homology between humans and cattle, since most of the CATs primers remain untested in cattle and consequently are unmapped. Thus any primers discovered to work in buffalo by the Egyptian laboratory will have a high probability of working in cattle and can then be added to the cattle map. The net result of the collaboration to screen the CATs primers will be a more efficient screen than either laboratory can perform alone. This proposal meets the INT objective of supporting U.S.- foreign collaboration in areas that have shared benefits for both sides. This project is being supported under the US- Egypt Joint Fund Program, which provides grants to scientists and engineers in both countries to carry out these cooperative activities. ***