CAA: Analysis of Vertebrate Telomere Proteins Using Gene Targeting

9722790价格这个项目的目标是开发一种脊椎动物细胞培养系统，用于对端粒蛋白基因进行定向破坏。这种培养系统将使研究人员能够探索不同的端粒蛋白在端粒功能中所起的作用。脊椎动物端粒是大的核蛋白复合体，预计这些复合体由许多不同的蛋白质组成。将对TRF1基因进行定向干扰，并分析缺失的表型。TRF1基因是编码第一个脊椎动物端粒蛋白的基因。将使用具有异常高同源重组频率的鸡B细胞系。TRF1基因的突变版本将被用来补充空的表型，以便剖析和了解蛋白质的功能。这项授予卡罗琳·普莱斯博士的职业发展奖将使她获得研究脊椎动物端粒功能所需的技术专长和深入知识。这一休假经历将使她能够将自己实验室的研究计划从研究纤毛原生动物的端粒功能转向对脊椎动物的类似研究。

9722790 Price The goal of this project is to develop a vertebrate cell culture system for making targeted disruptions of telomere protein genes. This culture system will allow researchers to explore the role different telomere proteins play in telomere function. Vertebrate telomeres are large nucleoprotein complexes and these complexes are expected to be comprised of many different proteins. Targeted disruptions of the TRF1 gene, the gene encoding the first vertebrate telomere protein identified, will be made and the null phenotype analyzed. A chicken B-cell line that has an unusually high frequency of homologous recombination will be used. Mutant versions of the TRF1 gene will be used to complement the null phenotype in order to dissect and understand the function of the protein. This Career Advancement Award to Dr. Carolyn Price will allow her to gain the technical expertise and the in-depth knowledge necessary for a study of vertebrate telomere function. This sabbatical experience will allow her to switch her research program in her own laboratory from investigations into the function of telomeres in ciliated protozoa to similar studies in vertebrates.