Pattern Formation in the Wing of Drosophila

小行星9723564 近年来，大量的信息已经积累了有关的模式，在果蝇生长的附属物。 果蝇的基因和分子操作的多样性是无与伦比的。 有趣的是，果蝇中使用的许多信号通路在脊椎动物中是保守的，通常在非常相似的模式化情况下。 然而，我们对这些模式化事件的理解仍然存在巨大的差距，这个项目概述了三个正在进行的项目，试图填补其中的一些空白。 果蝇的翅膀是由翅盘发育而来的，翅盘是一个小的上皮囊。 在椎间盘发育的广泛增殖阶段，细胞谱系在很大程度上是不固定的，细胞利用细胞-细胞相互作用来确定它们的位置并采取适当的命运。 然而，隔室边界是这一规则的例外;这些作为谱系限制，防止椎间盘上皮中相邻细胞之间的混合。 该建议的第一个目的是研究区室化的基础，利用一些令人兴奋的新数据，这些数据表明了房室间，基于刺猬的信号在维持A/P边界中的作用。 因为有很多不了解的途径，刺猬信号从后室到前室，本建议的第二个目的是继续调查的作用，转移，Hh信号通路的推定成员。 最后，局部模式也依赖于更多的局部相互作用来定义特定的组织类型，例如分泌静脉增厚角质层的特化上皮细胞。 最后一个目的是研究脉-脉间亚部，特别是crossveinless基因的作用是指定前和后横脉。

9723564 Blair In recent years a great deal of information has been accumulated concerning patterning of growing appendages in Drosophila. The variety of genetic and molecular manipulations possible in Drosophila are unmatched. Interestingly, many of the signalling pathways used in Drosophila are conserved in vertebrates, often in very similar patterning situations. However, there are still huge gaps in our understanding of these patterning events, and this project outlines three ongoing projects seeking to fill some of those gaps. The wing of Drosophila develops from the wing imaginal disc, a small epthelial sac. During the extensive proliferative phase of disc development cell lineages are for the most part unfixed and cells utilized cell-cell interactions to determine their positions and take on appropriate fates. The compartment boundaries, however, are exceptions to this rule; these act as lineage restrictions, preventing intermixing between adjacent cells in the disc epithelium. The first aim of this proposal is to examine the basis of compartmentalization, exploiting some exciting new data which indicates a role for intercompartmental, Hedgehog based signalling in maintaining the A/P boundary. Because there is much not understood about the pathway by which Hedgehog signals from the posterior to the anterior compartment, the second aim of this proposal is to continue investigating the role of shifted, a putative member of the Hh signalling pathway. Finally, local patterning also depends on more local interactions to define particular tissue types, such as the specialized epithelial cells that secrete the thickened cuticle of the veins. The last aim of this proposal is to examine the vein-intervein subdivision, and in particular the role of crossveinless genes is specifying the anterior and posterior cross veins.