Mechanisms Underlying Plant Cell Development
Mechanisms Underlying Plant Cell Development
批准号:
9723895
负责人:
James O'Leary
金额:
$22.5万
依托单位:
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-07-15 至 1999-06-30
中文摘要
小行星9723895 细胞分化的过程对我们理解 植物的生长发育,但在这方面的实验研究很少。 在发育过程中,每个细胞必须以某种方式确定其相对于其他细胞的位置, 因此必须加以区分。然而,几乎没有什么是已知的机制, 细胞通过这些细胞建立它们的位置并随后产生适当的细胞类型。 虽然细胞和组织分化肯定是选择性表达的结果, 一个关键的问题是我们无法将基因组中 表型和基因型。在基因结构甚至功能的知识 似乎呈指数级增长,但人们对这些基因如何 实际上导致细胞分化并形成组织、器官和有机体。 决定是个体细胞分化状态的反映。为此 因此,研究那些表现出高度决心的细胞可能是非常有用的, 如对再分化的抗性所示。植物表皮细胞就是这样的实体。在 在没有创伤的情况下(并且通常尽管有创伤),植物表皮细胞是 发育不全无法重新分化或改变命运。然而,融合花香 器官是这一规则的自然例外。 这里描述的工作的总体目标是更多地了解植物细胞的控制 分化这些实验代表了正在进行的细胞间 通信事件和传送位置信息的信号。具体 要解决的问题将集中在表征的化学特性的 再分化因子 这里描述的长春花系统是仅有的几个植物系统之一, 以扩散因子的形式记录细胞间相互作用的实验证据, 被展示。这也是第一个明确的文件,在一个工厂系统,两个显然 同一朵花中的相同器官可能与一朵花有本质上的和关键性的不同。 另
英文摘要
9723895 Verbeke The process of cellular differentiation is of fundamental significance to our understanding of plant growth and development, yet experimental studies in this area have been rare. During development, each cell must somehow determine its position relative to others, and must differentiate accordingly. However, almost nothing is known about the mechanisms by which cells establish their positions and subsequently give rise to appropriate cell types. Although cell and tissue differentiation must certainly be the result of selective expression of the genome, a critical problem has been our inability to make the connection between phenotype and genotype. At a time when knowledge of gene structure and even function seems to be increasing exponentially, there is still little known about how these genes actually cause cells to differentiate and form tissues, organs, and organisms. Determination is a reflection of the state of differentiation of individual cells. For this reason, it may be quite useful to study cells which display a high degree of determination, as indicated by a resistance to redifferentiate. Plant epidermal cells are such entities. In the absence of wounding (and usually in spite of wounding), plant epidermal cells are developmentally incompetent to redifferentiate or change their fate. However, fusing floral organs are naturally occurring exceptions to this rule. The overall goal of the work described here is to learn more about the controls of plant cell differentiation. These experiments represent an ongoing characterization of the cell-to-cell communication events and signals which convey positional information. The specific question to be addressed will focus on characterization of the chemical identity of the redifferentiation factors. The periwinkle system described here is one of only a few plant systems in which dire ct experimental evidence documenting cell-cell interaction in the form of diffusible factors has been shown. This is also the first clear documentation in a plant system that two apparently identical organs in the same flower may be inherently and critically different from one another.
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依托单位:
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