Characterization of Higher Plant Phosphoinositide-specific Phospholipase C

摘要9728024作物对环境变化的感知和反应的生化机制知之甚少。这位研究人员认为，肌醇磷脂的分解将环境信息带入植物细胞。磷脂酰肌醇分解产生肌醇磷酸盐和甘油二酯，它们在调节细胞生理方面起着关键作用。磷脂酰肌醇特异性磷脂酶C(PLC)在动物细胞的激素和环境信号转导中起关键作用。植物细胞也含有磷脂酰肌醇特异性的PLC，有证据表明这些酶参与了植物细胞对环境刺激的反应。虽然这些酶中的一些已经部分纯化，一些已经被克隆，但人们对这些酶的性质或调节，或者植物细胞中存在多少PLC知之甚少。这里描述的实验将研究这类酶，以了解它们在植物对环境信号的反应中的调节和作用。这些酶将在燕麦根中进行鉴定、纯化、测序和克隆，并研究它们的调控。这项研究将鉴定、纯化和研究植物中磷脂酶C酶的调节，磷脂酶C酶分解被称为磷脂酰肌醇的脂类，被认为在感知和响应环境刺激方面发挥重要作用。***

Abstract 9728024 Crain The biochemical mechanisms by which plants perceive and respond to changes in the environment are poorly understood. This investigator believes that the breakdown of phosphoinositide lipids brings environmental information into the plant cell. Phosphoinositide breakdown produces inositol phosphates and diacylglycerol, which play key roles in regulating cell physiology. Phosphoinositide specific phospholipases C (PLC) are critical in the transduction of hormonal and environmental signals in animal cells. Plant cells also contain PLCs specific for phosphoinositides, and there is evidence that these enzymes are involved in mediating plant cell response to environmental stimuli. Although some of these enzymes have been partially purified and some cloned, little is known about the properties or regulation of these enzymes, or how many PLCs are present in plant cells. The experiments described here will investigate this class of enzymes in order to understand their regulation and role in plant responses to environmental signals. The enzymes will be identified in oat roots and purified, sequenced and cloned, and their regulation examined. The research described here will identify, purify and study the regulation of phospholipase C enzymes in plants, which breakdown lipids known as phosphoinositides, and which are thought to play an important role in the perception of and response to environmental stimuli. ***